PARG1 Protein (AA 1-1266) (Strep Tag)

Produktnummer ABIN3136450

Produktdetails

Target: PARG1 (ARHGAP29) (rho GTPase Activating Protein 29 (ARHGAP29))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1266

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses PARG1 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequenz: MIAHKQKKAK KKRVWASGQP SAAITTSEMG LKSVSSSSSF DPEYIKELVN DVRKFSHMLL YLKEAILSDC FKEVIHIRLD ELLRVLKSIL SKHQNLSSVD LQSAAEVLTA KVKAVNFTEV NEENKNDIFR EVFSSIETLA FTFGNILTNF LMGDVGSDSI LRLPISRESK SFENISVDSV DLPHEKGNFS PIELDNLLLK NTDSIELALS YAKTWSKYTK NIVSWVEKKL NLELESTRNI VKLAEATRSS IGIQEFMPLQ SLFTNALLSD IHSSHLLQQT IAALQANKFV QPLLGRKNEM EKQRKEIKDL WKQQQNKLLE TETALKKAKL LCMQRQDEYE KAKSSMFRAE EEQLSSSVGL AKNLNKQLEK RRRLEEEALQ KVEEANEHYK VCVTNVEERR NDLENTKREI LTQLRTLVFQ CDLTLKAVTV NLFHMQQLQA ASLANSLQSL CDSAKLYDPG QEYSEFVKAT SSSELEEKVD GNVNKQMTNS PQTSGYEPAD SLEDVARLPD SCHKLEEDRC SNSADMTGPS FVRSWKFGMF SDSESTGGSS ESRSLDSESI SPGDFHRKLP RTPSSGTMSS ADDLDEREPP SPSEAGPNSL GAFKKTLMSK AALTHKFRKL RSPTKCRDCD GIVMFPGVEC EECLLVCHRK CLENLVIICG HQKLQGKMHI FGAEFIQVAK KEPDGIPFVL KICASEIENR ALCLQGIYRV CGNKIKTEKL CQALENGMHL VDISEFSSHD ICDVLKLYLR QLPEPFILFR LYKEFIDLAK EIQHVNEEQE AKKDSPEDKK HPHVSIEVNR ILLKSKDLLR QLPASHFNSL HYLIAHLRRV VDHAEENKMN SKNLGVIFGP TLIRPRPTTA PVTISSLAEY SNQARLVEFL ITYSQKIFDG SLQPQAVVIS NTGAVAPQVD QGYLPKPLLS PDERDTDHSM KPLFFSSKED IRSSDCESKS FELTTSFEES ERRQNALGKC DAPLLDNKVH LLFDQEHESA SQKMEDVCKS PKLLLLKSNR AANSVQRHTP RTKMRPVSLP VDRLLLLASS PTERSSRDVG NVDSDKFGKN PAFEGLHRKD NSNTTRSKVN GFDQQNVQKS WDTQYVRNNF TAKTTMIVPS AYPEKGLTVN TGNNRDHPGS KAHAEPARAA GDVSERRSSD SCPATAVRAP RTLQPQHWTT FYKPPNPTFS VRGTEEKTAL PSIAVPPVLV HAPQIHVTKS DPDSEATLAC PVQTSGQPKE SSEEPALPEG TPTCQRPRLK RMQQFEDLED EIPQFV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PARG1 (ARHGAP29) (rho GTPase Activating Protein 29 (ARHGAP29))

Andere Bezeichnung: Arhgap29 (ARHGAP29 Produkte)

Synonyme: arhgap29 Protein, sb:cb905 Protein, zgc:63950 Protein, ARHGAP29 Protein, PARG1 Protein, RP11-255E17.1 Protein, 6720461J18Rik Protein, A830014I19 Protein, AU040217 Protein, B130017I01Rik Protein, C76601 Protein, Parg1 Protein, B130017i01rik Protein, RGD1306185 Protein, Rho GTPase activating protein 29 Protein, Rho GTPase activating protein 29b Protein, Rho GTPase activating protein 29 S homeolog Protein, ARHGAP29 Protein, arhgap29b Protein, arhgap29.S Protein, arhgap29 Protein, Arhgap29 Protein

Hintergrund: Rho GTPase-activating protein 29 (Rho-type GTPase-activating protein 29),FUNCTION: GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state. Has strong activity toward RHOA, and weaker activity toward RAC1 and CDC42. May act as a specific effector of RAP2A to regulate Rho (By similarity). In concert with RASIP1, suppresses RhoA signaling and dampens ROCK and MYH9 activities in endothelial cells and plays an essential role in blood vessel tubulogenesis. {ECO:0000250, ECO:0000269|PubMed:21396893}.

Molekulargewicht: 142.3 kDa

UniProt: Q8CGF1