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SMC5 Protein (AA 1-1101) (His tag)

Crystallography grade SMC5 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3136442
  • Target Alle SMC5 Proteine anzeigen
    SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1101
    Spezies
    Maus
    Quelle
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses SMC5 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MATPSGKAAP PNPQVSKRSL PRDASSEVPS KRKNSNPLPT LPRPSGTFVE GSIVRIAMEN FLTYDICEVS PGPHLNMIIG ANGTGKSSIV CAICLGLAGK PAFMGRADKV GFFVKRGCSK GLVEIELFRT SGNLIITREI DVIKNQSFWF INKKPVTQKI VEEQVAALNI QVGNLCQFLP QDKVGEFAKL SKIELLEATE KSVGPPEMHR YHCELKNFRE KEKQLETSCK EKTEYLEKMV QRNERYKQDV ERFYERKRHL DLIEMLEAKR PWVEYENVRQ EYEGVKLIRD RVKEEVRKLK EGQIPMTRRI EEIDRQRHTL EVRIKEKSTD IKEASQKCKQ RQDLIERKDR QIKELQQALT VKQNEELDRQ KRISNTRKMI EDLQSELKTA ENCENLQPQI DTVTNDLRRV QEEKALCEGE IIDKQREKEM LEKQRRSVSD HITRFDNLMN QKEDKLRQRY RDTYDAVLWL RNNRDRFKQR VCEPIMLTIN MKDNKNAKYV ENHISSNDLR AFVFESQEDM EIFLREVRDN KKLRVNAVIA PKISYADKAP SRSLNDLKQY GFFSYLRELF DAPDPVMSYL CCQYHIHEVP VGTERTRERI ERVIQETRLK QIYTAEEKYV LKTSVYSNKV ISSNTSLKVA QFLTVTVDLE QRRHLEEQLK EMNRQLEAVD SGLAALRDTN RHLELKDNEL RLKKKELLER KTRKRQLEQK ISSKLASIRL MEQDTCNLEE EERKASTKIK EINVQKAKLV TELTGLVKIC TSFQIQKVDL ILQNTTVISE KNKLEADYMA SSSQLRVTEQ QFIELDDNRQ RLLQKCKELM KKARQVCNLS ADQAVPQEFQ TQVPTIPNGH SSSPPMAFQD LPNTLDEIDA LLTEERSRAS CFTGLNPSVV EEYSKREVEI QQLTEELQGK KVELDEYREN ISQVKERWLN PLKELVEKIN EKFSNFFSSM QCAGEVDLHT ENEEDYDKYG IRIRVKFRSS TQLHELTPHH QSGGERSVST MLYLMALQEL NRCPFRVVDE INQGMDPINE RRVFEMVVNT ACKENTSQYF FITPKLLQNL PYSEKMTVLF VYNGPHMLEP NRWNLKAFQR RRRRITFTQP Q
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Smc5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product SMC5 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    SMC5 (Structural Maintenance of Chromosomes 5 (SMC5))
    Andere Bezeichnung
    Smc5 (SMC5 Produkte)
    Synonyme
    F1N13.60 Protein, F1N13_60 Protein, structural maintenance of chromosomes 5 Protein, SMC-5 Protein, SMC5L1 Protein, MLZ-453 Protein, Smc5l1 Protein, mKIAA0594 Protein, mSMC5 Protein, structural maintenance of chromosomes 5 Protein, structural maintenance of chromosomes 5 S homeolog Protein, SMC5 Protein, smc5 Protein, Smc5 Protein, smc5.S Protein
    Hintergrund
    Core component of the SMC5-SMC6 complex, a complex involved in repair of DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination and mediates sumoylation of shelterin complex (telosome) components. Required for sister chromatid cohesion during prometaphase and mitotic progression, the function seems to be independent of SMC6 (By similarity). {ECO:0000250}.
    Molekulargewicht
    129.8 kDa Including tag.
    UniProt
    Q8CG46
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