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MYT1 Protein (AA 1-1127) (His tag)

Crystallography grade MYT1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3136430
  • Target Alle MYT1 Proteine anzeigen
    MYT1 (Myelin Transcription Factor 1 (MYT1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1127
    Spezies
    • 5
    • 1
    Maus
    Quelle
    • 3
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses MYT1 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MSSESDDKRA RTRSKTLRGP PETTGADLSC PTPGCTGSGH VRGKYSRHRS LQSCPLAKKR KLEDAETEHL VSKRKSHPLR LALDEGYRMD SDGSEDAEVK DVSVSDESEG PLEEAEAEMS GQEEIHHPQT AEGKSLIKPH FDSNPTSSPS GFSKSSYSSY QGIIATSLLN LGQIAEEALV KEDSVSVAKL SPTVVHQLQD EAAMGVNSDE GEKDLFIQPE DVEEVIEVTS ERSQEPCPQS LKDMVSEESS KQKGVLGHEE EGEEEEEDEE EEDEEEEEEG EEGEEEEEEE EEEEEEEDEE EEEEEEEAAP NVIFGEDTSH TSVQKASPEF RGPELSSPKP EYSVIVEVRS DDDKDEDSRS QKSAVTDESE MYDMMTRGNL GLLEQAIALK AEQVRAVCES GCPPAEQGHL GPGEPGKMAK PLDVVRKSCY SKDPSRVEKR EIKCPTPGCD GTGHVTGLYP HHRSLSGCPH KDRIPPEILA MHENVLKCPT PGCTGQGHVN SNRNTHRSLS GCPIAAAEKL AKSHEKQQLQ TGDPPKNNSN SDRILRPMCF VKQLEVPPYG SYRPNVAPAT PRANLAKELE KFSKVTFDYA SFDAQVFGKR MLAPKIQTSE TSPKAFQCFD YSHDAEAAHM AATAILNLST RCWEMPENLS TKPQDLPSKA VDIEVDENGT LDLSMHKHRK RENTFPSSSS CSSSPGVKSP DVSQRQSSTS APSSSMTSPQ SSQASRQDEW DRPLDYTKPS RLREEEPEES EPAAHSFASS EADDQEVSEE NFEERKYPGE VTLTNFKLKF LSKDIKKELL TCPTPGCDGS GHITGNYASH RSLSGCPLAD KSLRNLMAAH SADLKCPTPG CDGSGHITGN YASHRSLSGC PRAKKSGLKV APTKDDKEDP ELMKCPVPGC VGLGHISGKY ASHRSASGCP LAARRQKEGA LNGSSFSWKS LKNEGPTCPT PGCDGSGHAN GSFLTHRSLS GCPRATFAGK KGKLSGDEIL SPKFKTSDVL ENDEEIKQLN QEIRDLNESN SEMEAAMVQL QSQISSMEKN LKNIEEENKL IEEQNEALFL ELSGLSQALI QSLANIRLPH MEPICEQNFD AYVNTLTDMY SNQDCYQNPE NKGLLETIKQ AVRGIQV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Myt1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MYT1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    MYT1 (Myelin Transcription Factor 1 (MYT1))
    Andere Bezeichnung
    Myt1 (MYT1 Produkte)
    Synonyme
    fc56h05 Protein, myt1 Protein, si:dkey-202m22.5 Protein, wu:fc56h05 Protein, C20orf36 Protein, MTF1 Protein, MYTI Protein, PLPB1 Protein, ZC2HC4A Protein, Nzf2 Protein, Nzf2a Protein, Nzf2b Protein, Nztf2 Protein, myelin transcription factor 1 Protein, myelin transcription factor 1a Protein, myelin transcription factor 1 S homeolog Protein, MYT1 Protein, myt1a Protein, Myt1 Protein, myt1.S Protein
    Hintergrund
    Binds to the promoter region of genes encoding proteolipid proteins of the central nervous system. May play a role in the development of neurons and oligodendroglia in the CNS. May regulate a critical transition point in oligodendrocyte lineage development by modulating oligodendrocyte progenitor proliferation relative to terminal differentiation and up-regulation of myelin gene transcription (By similarity). {ECO:0000250, ECO:0000269|PubMed:9373037}.
    Molekulargewicht
    124.5 kDa Including tag.
    UniProt
    Q8CFC2
    Pathways
    Zellzyklus
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