DEF6 Protein (AA 1-630) (Strep Tag)

Produktnummer ABIN3136367

Produktdetails

Target: DEF6 (Differentially Expressed in FDCP 6 Homolog (DEF6))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-630

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses DEF6 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MALRKELLKS IWYAFTALDV EKSGKVSKSQ LKVLSHNLYT VLNIPHDPVA LEEHFRDDDD GPVSSQGYMP YLNKYILDKV EEGAFVKEHF DELCWTLTAK KNYRADGIGS SPLSNQDAFR LWCLFNFLSE DKYPLIMVPD EVEYLLKKLL GSLSLEMGLG ELEELLAQDA QSAQTAVGLS VWQFLELFNS GRCLRGVGRD SLSMAIQEVY QELIQDVLKQ GYLWKRGHLR RNWAERWFQL QPSSLCYFGS EECKEKRGTI PLDAHCCVEV LPDREGKRCM FCVKTASRTY EMSASDTRQR QEWTAAIQTA IRLQAEGKTS LHKDLKQKRR EQREQRERRR AAKEEELLRL QQLQEEKERK LQELELLQEA QRQAERLLQE EEERRRSQHK ELQQALEGQL REAEQARASM QAEMELKKEE AARQRQRIAE LEEMQERLQE ALQLEVKARR DEEAVRLAQT RLLEEEEEKL KQLMHLKEEQ ERYIERAQQE KQELQQEMAL QSRSLQHAQQ QLEEVRQNRQ RADEDVEAAQ RKLRQASTNV KHWNVQMNRL MHPIEPGDKR PTTSSSFTGF QPPPLARRDS SLKRLTRWGS QGNRTLSVNS SEQKSLNGGD ETPILALASQ EEKLDPAPGN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DEF6 (Differentially Expressed in FDCP 6 Homolog (DEF6))

Andere Bezeichnung: Def6 (DEF6 Produkte)

Synonyme: DEF6 Protein, Def6 Protein, zgc:63721 Protein, ibp Protein, slat Protein, swap70l Protein, MGC68775 Protein, 2410003F05Rik Protein, 6430538D02Rik Protein, AV094905 Protein, Ibp Protein, Slat Protein, Slat2 Protein, Slat6 Protein, IBP Protein, SLAT Protein, SWAP70L Protein, DEF6, guanine nucleotide exchange factor Protein, differentially expressed in FDCP 6a homolog (mouse) Protein, DEF6, guanine nucleotide exchange factor L homeolog Protein, differentially expressed in FDCP 6 Protein, DEF6 guanine nucleotide exchange factor Protein, DEF6 Protein, def6a Protein, def6.L Protein, def6 Protein, Def6 Protein

Hintergrund: Differentially expressed in FDCP 6 (DEF-6) (IRF4-binding protein) (SWAP-70-like adapter of T-cells),FUNCTION: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which plays a role in the activation of Rho GTPases RAC1, RhoA and CDC42 (PubMed:12648457, PubMed:12923183). Can regulate cell morphology in cooperation with activated RAC1 (PubMed:12648457, PubMed:12923183). Involved in immune homeostasis by ensuring proper trafficking and availability of T-cell regulator CTLA-4 at T-cell surface (By similarity). Plays a role in Th2 (T helper cells) development and/or activation, perhaps by interfering with ZAP70 signaling. Required for optimal T-cell effector function, lymphocyte homeostasis and the prevention of systemic autoimmunity (By similarity). {ECO:0000250|UniProtKB:Q9H4E7, ECO:0000269|PubMed:12648457, ECO:0000269|PubMed:12923183}.

Molekulargewicht: 73.5 kDa

UniProt: Q8C2K1