GEN1 Protein (AA 1-908) (Strep Tag)

Produktnummer ABIN3136234

Produktdetails

Target: GEN1 (Gen Endonuclease Homolog 1 (GEN1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-908

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses GEN1 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequenz: MGVNDLWQIL EPVKQHIHLQ DLSGKTIAVD LSLWVCEAQT VKKMIGTVKK PHLRNLFFRI SYLTQMNVKL VFVMEGEPPM LKADVISKRT QTRYGPSGKS RSQKTGRSHF KSVLRECLEM LECLGMPWVQ AAGEAEAMCA YLNASGHVDG CLTNDGDAFL YGAQTVYRNF TMNTKDPHVD CYTISSIKSK LGLDRDALVG LAVLLGCDYL PKGVPGVGKE QALKLLQIFK GQSLLQRFNQ WIEDPCYSVP QSAPKKVVHC SVCSHPGSPK DHERNGCILC KSDKYCEPHD YDYLCPCEWH QTDHNRHLSE IENNIKKKAC SCEGFPFHEV IQEFLLNKNK MLKPITYQRP DLLLFQRFTV QKMEWPSHYA CEKLLVLLTR YDMIERKHGR KTSNQLQPIR IVKPRVRNGV HCLEIEWEKP EHYVVEDGDP GKLSLLTMEE ASLFEAAYPD AVAVYQKQLS ETKGRKQKSM KNKPKGSHLP EADDVINSQS LMTLKPTSKA FPKQNPKINL ENSPDPILAQ ESTSPSLNSF VSPENAPCLN LQEQLVPSPR TLAIKQSKDV SHFLVSECSQ PSSSSHDISV ITDLQLSTID WAGTSFSNSP AVQRNTFSQD LASESESSAI LPDFEQLSYE SEQGTSDSEG SGRDLQQSNP EEQLLSGISA LHLHDLPLKE RIRIKSSCPQ YNVGADAGLE SLPLKLKGSC IAYSSSDGSS NFSKDLTGVY LHKESRNSKV LDSRLQENCG ANTSLPYSFS DKAVKTSSLQ VGLPTAAIPH NPRVAVKTTK NLVMKNSVCL ERDSSDEDNA PGSWKSKYTA PEMKHSSQKH SLVHVRDSTH NKLRNPKVES KETKLCNESF KTAEDEENGF SDLGRSPQSF RPCHDKDENS TASWENPLPL RQRLKLRFQN TQSGFYNT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: GEN1 (Gen Endonuclease Homolog 1 (GEN1))

Andere Bezeichnung: Gen1 (GEN1 Produkte)

Synonyme: 5830483C08Rik Protein, Gen Protein, RGD1559792 Protein, GEN1, Holliday junction 5' flap endonuclease Protein, GEN1 Holliday junction 5' flap endonuclease Protein, GEN1 Protein, Gen1 Protein

Hintergrund: Flap endonuclease GEN homolog 1 (EC 3.1.-.-),FUNCTION: Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation. Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction. Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA. Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates. {ECO:0000250|UniProtKB:Q17RS7}.

Molekulargewicht: 101.8 kDa

UniProt: Q8BMI4

Pathways: DNA Reparatur