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CEMIP Protein (AA 31-1361) (His tag)

Crystallography grade CEMIP Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3136173
  • Target Alle CEMIP Proteine anzeigen
    CEMIP (Cell Migration Inducing Hyaluronan Binding Protein (CEMIP))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 31-1361
    Spezies
    Maus
    Quelle
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses CEMIP Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    AVATECPDQS PELQPWSPGH NRDYQVHIGH GRKLLLTSSA TVHSITISGG GKLVIKDHHE HIVLRTRYIL IDDGGELHAG SALCPFEGNF SIVLYGRADE NILPDPYYGL KYIGVDKGGT LELHGQKKLS WTFLNKTLHP GGMQEGGYFF ERSWGHRGVI VHVIDAKLGT VVHSDRFDTY RSKKESERLV QYLNAVPDGR ILSVAVNDEG SRNLDDTARK AMTKLGSKHF LHLGFRHPWS FITVKGNPSS SVEDHIEYHG HKGSAAARVF KLFQTEHGEH FNVSSSSEWV QDVEWTEWFD HDKVPQSKGG EKISDLRAAY PGKICNRPID IQATTMDGVA LSTEVVYKNG QDYRFACYTR GRACRSYRVR FLCGKPVRPK LTVSIDTNVN STILSLVDNV RSWRPGDTLV VASTDYSMYQ AEEFRVLPCK ACTSTQVKVA GKPQYLHIGE EIDGVDMRAE VGLLTRNIVV MGEMEDRCYP YTNHICDFFD FDTFGGHIKF ALGFKAAHLE GVELKYMGQQ LVGQYPIHFH LAGDLDEQGG YDPPTYIRDL SIHHTFSRCI TVHGSNGLLI KDVVGYNSLG HCFFTEDGPE ERNTFDHCLG LLVKSGTLLP SDRDSRMCKV ITEDSYPGYI PKPRQDCNAV STFWMANPNN NLINCAAAGS EETGFWFIFH HVPTGPSVGM YSPGYSEHIP LGKFYNNRAH SNYRAGMIID NGVKTTEASA KDKRPFLSII SARYSPHQDA DPLKPREPAI IRHFTAYKNQ DHGAWLRGGD VWLDSCRFAD NGIGLTLASG GTFPYDDGSK QEIKNSLFVG ESGNVGTEMM DNRIWGPGGL DHSGRTLPIG QNFPIRGIQF YDGPINIQNC TFRKFAALEG RHTSALAFRL NNAWQSCPHN NVTNIAFEDV PITSRVFFGE PGPWFNQLDM DGDKTSVFHD LDGSVSEYPG SYLTKDDNWL VRHPDCINVP DWRGAICSGR YAQMYIQAYK SSNLRMKIIK NDFPSHPLYL EGALTRSTHY QQYQPVITLQ KGYTIHWDQT APAELAIWLI NFNKGDWIRV GLCYPRGTTF SILSDVHNRL LKQTSKTGTF VRTLQMDKVE QSYPGRSHYY WDEDSGLLFL KLKAQNEREK FAFCSMKGCE RIKIKALLPR NAGISDCTAT AYPRFTERAI VDVPMPRKLF GAQLKTKDHF LEVKMESSRQ HFFHLRNDFA YIEVDGRRYP CSEDGIQIVV IDGSRGHVVS HGSFRNAILQ GIPWQLFNYV AAIPDNSIVL MASKGRYITR GPWTRVLEKL GADKGLKLKE KMAFVGFKGS FRPIWVTLET EDHKAKIFQV VPIPVVRKKK L
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Cemip Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CEMIP Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CEMIP (Cell Migration Inducing Hyaluronan Binding Protein (CEMIP))
    Andere Bezeichnung
    Cemip (CEMIP Produkte)
    Synonyme
    CCSP1 Protein, TMEM2L Protein, 3110012M15Rik Protein, mKIAA1412 Protein, 12H19.01.T7 Protein, 6330404C01Rik Protein, AY007814 Protein, Kiaa1199 Protein, KIAA1199 Protein, cell migration inducing hyaluronan binding protein Protein, transmembrane protein 2 Protein, cell migration inducing protein, hyaluronan binding Protein, CEMIP Protein, Tmem2 Protein, TMEM2 Protein, Cemip Protein
    Hintergrund
    Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing. {ECO:0000269|PubMed:24251095}.
    Molekulargewicht
    150.9 kDa Including tag.
    UniProt
    Q8BI06
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