NUP107 Protein (AA 1-926) (Strep Tag)

Produktnummer ABIN3136158

Produktdetails

Target: NUP107 (Nucleoporin 107kDa (NUP107))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-926

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses NUP107 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MDRSGFGGMS SPVIRDAEVT RTARKHSAHK RVLIQANQED NFGTATPRSQ IIPRTPSSFR QPFVTPSSRS LLRHPDISYI LGTEGRSPRH TQSSGYLGNL SMVTNLDDSN WAAAFSSQRL GLYTNTEHHS MTEDVNLSTV MLREDDPGEA ASMSMFSDFL HSFLKHSSTT VFDLVEEYEN ICGSQVNILS KIVSRATPGL QKFSKTASML WLLQQEMVTW RLLASLYRDR IQSSLEEENM FAIAGINASE KMVVETLFQR DSLVRQSQLV VDWLESIAKD EIGEFSDNIE FYAKSVYWEN TLHSLKQRQL LSHMGSTRPL VTELDPDAPI RQKLPLDDLD REDEVRLLKY LFTLIRAGMT EEAQRLCKRC GQAWRAATLE GWKLYHDPNV NGGTELEPVE GNPYRRIWKI SCWRMAEDEL FNKYERAIYA ALSGNLKQLL PVCDTWEDTV WAYFRVMVDS LVEQEIRTSV MTQDDSEELP REYMEANWTL EKVFEELQAT DKKRVLEENQ EHYHIVQKFL ILGDVDGLMD EFSKWLSKSG SSLPGHLLRF MTHLILFLRT LGLQTKEEVS IEVLKTYIQL LISEKHTSLI AFYTCHLPQD LAVAQYALFL EGVTEFEQRH QCLELAKEAD LDVATITKTV VENICKKDNG EFSHHDLAPS LDTGTTEEDR LKIDVIDWLV FDPAQRAEAL RQGNAIMRKF LALKKHEAAK EVFVKIPQDS IAEIYNQWEE QGMESPLPAE DDNAIREHLC IRAYLEAHET FNEWFKHMNS APQKPTLLSQ ATFTEKVAYE HREKKYEMDH NIWKGHLDAL TADVKEKMYN VLLFVDGGWM VDVREDAEDD PERTHQMVLL RKLCLPMLCF LLHTILHSTG QYQECLQLAD MVSSERHKLY LVFSKEELRK LLQKLRESSL MLLDQGLDPL GYEIQS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NUP107 (Nucleoporin 107kDa (NUP107))

Andere Bezeichnung: Nup107 (NUP107 Produkte)

Synonyme: zgc:152723 Protein, si:dkey-27k7.3 Protein, Afu1g10860 Protein, BcDNA:LD18761 Protein, CG6743 Protein, Dmel\\CG6743 Protein, Nup 107 Protein, Nup170 Protein, dmNup107 Protein, nup107 Protein, SPBC582.11c Protein, AW541137 Protein, C76801 Protein, NUP84 Protein, nucleophosmin/nucleoplasmin 3 Protein, nucleoporin 107 Protein, nuclear pore complex protein Nup107 Protein, Nuclear pore complex protein Nup107 Protein, nucleoporin 107kDa S homeolog Protein, nucleoporin 107kDa Protein, Nucleoporin 107kD Protein, nucleoporin Nup107 Protein, NPM3 Protein, NUP107 Protein, nup107 Protein, AFUA_1G10860 Protein, LOC5563712 Protein, CpipJ_CPIJ003997 Protein, BDBG_00235 Protein, nu107 Protein, nup107.S Protein, Nup107 Protein

Hintergrund: Nuclear pore complex protein Nup107 (107 kDa nucleoporin) (Nucleoporin Nup107),FUNCTION: Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. Involved in nephrogenesis. {ECO:0000250|UniProtKB:P57740}.

Molekulargewicht: 106.7 kDa

UniProt: Q8BH74

Pathways: Protein targeting to Nucleus