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ATG4D Protein (AA 64-474) (His tag)

Crystallography grade ATG4D Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3136149
  • Target Alle ATG4D Proteine anzeigen
    ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 64-474
    Spezies
    • 3
    • 1
    • 1
    Maus
    Quelle
    • 3
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ATG4D Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    KFKAKFLTAW NNVKYGWAVK SRTSFSKIST VHLCGRCYHF EGEGDIQRFQ RDFVSRLWLT YRRDFPPLAG GSLTSDCGWG CMLRSGQMML AQGLLLHFLP RDWRWVEGTG LASSEMPGPA SPSRCRGPGR RGPPRWTQGA LEMEQDRWHR RIVSWFADHP RAPFGLHRLV ELGRSSGKKA GDWYGPSVVA HILRKAVESC SEVSRLVVYV SQDCTVYKAD VARLLSWPDP TAEWKSVVIL VPVRLGGETL NPVYVPCVKE LLRSELCLGI MGGKPRHSLY FIGYQDDFLL YLDPHYCQPT VDVSQPSFPL ESFHCTSPRK MAFAKMDPSC TVGFYAGNRK EFETLCSELM RILSSSSVTE RYPMFTVAEG HAQDHSLDAL CTQLSQPTLR LPCTGRLLKA KRPSSEDFVF L
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Atg4d Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ATG4D Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ATG4D (Autophagy related 4D Cysteine Peptidase (ATG4D))
    Andere Bezeichnung
    Atg4d (ATG4D Produkte)
    Synonyme
    APG4-D Protein, APG4D Protein, AUTL4 Protein, 9830134P12Rik Protein, Apg4d Protein, Atg4dl Protein, Autl4 Protein, apg4d Protein, autophagy related 4D cysteine peptidase Protein, autophagy related 4D, cysteine peptidase Protein, autophagy related 4D, cysteine peptidase L homeolog Protein, ATG4D Protein, Atg4d Protein, atg4d.L Protein
    Hintergrund
    Cysteine protease ATG4D: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. {ECO:0000250|UniProtKB:Q86TL0}., Cysteine protease ATG4D, mitochondrial: Plays a role as an autophagy regulator that links mitochondrial dysfunction with apoptosis. The mitochondrial import of ATG4D during cellular stress and differentiation may play important roles in the regulation of mitochondrial physiology, ROS, mitophagy and cell viability (By similarity). {ECO:0000250}.
    Molekulargewicht
    47.5 kDa Including tag.
    UniProt
    Q8BGV9
    Pathways
    Autophagie
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