PAN2 Protein (AA 1-1200) (Strep Tag)

Produktnummer ABIN3136135

Produktdetails

Target: PAN2 (PAN2 Poly(A) Specific Ribonuclease Subunit Homolog (PAN2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1200

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses PAN2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MNFEGLDPGL AEFSPAMHST LDPVLDAHLN PSLLQNVELD PEGVALEALP VQESVHIMEG VYSELHSVVA EVGVPVSVSH FDLHEEMLWV GSHGGHATSF FGPALERYSS FQVNGGDDIR QIQSLENGIL FLTKNNLKYM ARGGLIIFDY LLDENEDMHS VLLTDNSTLL VGGLQNHVLE IDLNTVQETQ KYAVETPGVT IMRQTNRFFF CGHTSGKVSL RDLRSFKVEH EFDAFSGSLS DFDVHGNLLA ACGFSSRLTG LACDRFLKVY DLRMMRAITP LQVHVDPAFL RFIPTYTSRL AIISQSGQCQ FCEPTGLANP ADIFHVNPVG PLLMTFDVSA SKQALAFGDS EGCVHLWTDS PEPSFNPYSR ETEFALPCLV DSLPPLDWSQ DLLPLSLIPV PLTTDALLSD WPAANSAPAP RRAPPVDAEI LRTMKKVGFI GYAPNPRTRL RNQIPYRLKE SDHEFDNFSQ VTESPTGREE EPLHTVSKKY RKVTIKYSKL GLEDFDFKHY NKTLFAGLEP HIPNAYCNCM IQVLYFLEPV RCLIQNHLCQ KEFCLACELG FLFHMLDLSR GDPCQGSNFL RAFRTIPEAS ALGLILADSD EASGKGSLAR LIQRWNRFIL TQLHQDMQEL EVPQAYRGAG GSFCSSGDSI IGQLFSCEME NCSLCRCGSE TVRASSTLLF TLSYPEDKTG KNYDFAQVLK RSICLEQNTQ AWCDNCEKYQ PTIQTRNIRH LPDILVINCE VNSSKEADFW RLQAEVAFKI AVKKYGGEMK SKEFALADRK ELRSPEGFLC SSIEELKNVW LPFSIRMKMT KNKGLDVCNW ADEHELSSLG APSQWGPARA EEELGVYVYD LMATVVHILD SRTGGSLVAH IKVGETYHQR KEGVTHQQWY LFNDFLIEPI DKYEAVQFDM NWKVPAILYY VKRNLNSRYN LNIKNPIEAS VLLAEASLAR KQRKTHTTFI PLMLNEMPQV GDLVGLDAEF VTLNEEEAEL RSDGTKSTIK PSQMSVARIT CVRGQGPNEG IPFIDDYIST QEQVVDYLTQ YSGIKPGDLD AKISSKHLTT LKSTYLKLRF LIDIGVKFVG HGLQKDFRVI NLMVPKDQVL DTVYLFHMPR KRMISLRFLA WYFLDLKIQG ETHDSIEDAR TALQLYRKYL ELSKNGTEPE SFHKVLKGLY EKGRKMDWKV PEPESQTSPK NAAVFSVLAL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PAN2 (PAN2 Poly(A) Specific Ribonuclease Subunit Homolog (PAN2))

Andere Bezeichnung: Pan2 (PAN2 Produkte)

Synonyme: USP52 Protein, 1200014O24Rik Protein, AI047843 Protein, AW742773 Protein, Usp52 Protein, mKIAA0710 Protein, PAN2 Protein, pan2 Protein, PAN2 poly(A) specific ribonuclease subunit Protein, PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae) Protein, PAN2 poly(A) specific ribonuclease subunit S homeolog Protein, PAN2 Protein, Pan2 Protein, pan2.S Protein

Hintergrund: PAN2-PAN3 deadenylation complex catalytic subunit Pan2 (EC 3.1.13.4) (Inactive ubiquitin carboxyl-terminal hydrolase 52) (PAB1P-dependent poly(A)-specific ribonuclease) (Poly(A)-nuclease deadenylation complex subunit 2) (PAN deadenylation complex subunit 2),FUNCTION: Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 (PubMed:16284618). Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation (By similarity). {ECO:0000255|HAMAP-Rule:MF_03182, ECO:0000269|PubMed:16284618}.

Molekulargewicht: 135.3 kDa

UniProt: Q8BGF7