NrCAM Protein (AA 30-1119) (His tag)

Produktnummer ABIN3136101

Produktdetails

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Protein-Typ: Recombinant

Proteineigenschaft: AA 30-1119

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses NrCAM Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: LDVPLDLVQP PTITQQSPKD YIIDPRENIV IQCEAKGKPP PSFSWTRNGT HFDIDKDPLV TMKPGSGTLV INIMSEGKAE TYEGVYQCTA RNERGAAVSN NIVVRPSRSP LWTKERLEPI VLQNGQSLVL PCRPPIGLPP AIIFWMDNSF QRLPQSERVS QGLNGDLYFS NVLPEDTRED YICYARFNHT QTIQQKQPIS LKVISVDELN DTIAANLSDT EFYGAKSSKE RPPTFLTPEG NESHKEELRG NVLSLECIAE GLPTPIIYWI KEDGMLPANR TFYRNFKKTL QITHVSEADS GNYQCIAKNA LGAVHHTISV TVKAAPYWIV APQNLVLSPG ENGTLICRAN GNPKPRISWL TNGVPIEIAL DDPSRKIDGD TIIFSNVQES SSAVYQCNAS NKYGYLLANA FVNVLAEPPR ILTSANTLYQ VIANRPALLD CAFFGSPMPT IEWFKGTKGS ALHEDIYVLH DNGTLEIPVA QKDSTGTYTC VARNKLGMAK NEVHLEIKDP TRIIKQPEYA VVQRGSKVSF ECRVKHDHTL IPTIMWLKDN GELPNDERFS TDKDHLVVSD VKDDDGGTYT CTANTTLDSA SASAVLRVVA PTPTPAPIYD VPNPPFDLEL TNQLDKSVQL TWTPGDDNNS PITKFIIEYE DAMHDAGLWR HQAEVSGTQT TAQLKLSPYV NYSFRVMAEN SIGRSMPSEA SEQYLTKAAE PDQNPMAVEG LGTEPDNLVI TWKPLNGFQS NGPGLQYKVS WRQKDGDDEW TSVVVANVSK YIVSGTPTFV PYLIKVQALN DVGFAPEPAA VMGHSGEDLP MVAPGNVRVS VVNSTLAEVH WDPVPPKSVR GHLQGYRIYY WKTQSSSKRN RRHIEKKILT FQGTKTHGML PGLQPYSHYA LNVRVVNGKG EGPASTDRGF HTPEGVPSAP SSLKIVNPTL DSLTLEWDPP SHPNGILTEY ILQYQPINST HELGPLVDLK IPANKTRWTL KNLNFSTRYK FYFYAQTSVG PGSQITEEAI TTVDEAGIPP PDVGAGKGKE EWRKEIVNGS RSFFGLKGLM PGTAYKVRVG AEGDSGFVSS EDVFETGPAM ASRQVDIATQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Nrcam Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Andere Bezeichnung: Nrcam (NRCAM Produkte)

Synonyme: CD56 Protein, MSK39 Protein, NCAM Protein, Bravo Protein, C030017F07Rik Protein, C130076O07Rik Protein, mKIAA0343 Protein, NRCAM Protein, si:dkey-240a12.1 Protein, Nr-CAM Protein, neural cell adhesion molecule 1 Protein, neuronal cell adhesion molecule Protein, neuronal cell adhesion molecule L homeolog Protein, neuronal cell adhesion molecule a Protein, NCAM1 Protein, NRCAM Protein, Nrcam Protein, nrcam.L Protein, nrcama Protein, nrcam Protein

Hintergrund: Cell adhesion protein that is required for normal responses to cell-cell contacts in brain and in the peripheral nervous system. Plays a role in neurite outgrowth in response to contactin binding (PubMed:11564762). Plays a role in mediating cell-cell contacts between Schwann cells and axons (PubMed:20188654). Plays a role in the formation and maintenance of the nodes of Ranvier on myelinated axons. Nodes of Ranvier contain clustered sodium channels that are crucial for the saltatory propagation of action potentials along myelinated axons. During development, nodes of Ranvier are formed by the fusion of two heminodes. Required for normal clustering of sodium channels at heminodes, not required for the formation of mature nodes with normal sodium channel clusters (PubMed:14602817, PubMed:20188654). Required, together with GLDN, for maintaining NFASC and sodium channel clusters at mature nodes of Ranvier (PubMed:24719088). {ECO:0000269|PubMed:11564762, ECO:0000269|PubMed:14602817, ECO:0000269|PubMed:16039564, ECO:0000269|PubMed:20188654, ECO:0000269|PubMed:24719088}.

Molekulargewicht: 121.3 kDa Including tag.

UniProt: Q810U4

Pathways: Regulation of Cell Size