FGD5 Protein (AA 1-1219) (Strep Tag)

Produktnummer ABIN3136032

Produktdetails

Target: FGD5 (FYVE, RhoGEF and PH Domain Containing 5 (FGD5))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1219

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses FGD5 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MGSPESEVSP DVQEQEAATD NPEVFEEDSA DAAEGEDQIE QEEPPNCDEE AYNRDAAAAT MQVGEDLGEE GDHVQEDPAE ESCQIIPFES DSVEEDFSPT LTENPYEIFP TESTSFCNNT YSLDESANGH EPVCEICVEE VPGVGPPLNQ HDSLPDGSGE DSPVVPDVVV VPENEGPVDD ALSSPYVMGV GLLSLGEGAQ SDTQAASGTL SGYSTWEEGD SEGGQVPVDR KNIATRARPH SGKVAGHVPE TVLEETGPET CSSGMGIRDT SDEVRKIGIL PEGKPPECVR ALPAKPRAFT LYPRSFSVEG RESPLSMFRE PEGAGLDSHR VRRKEDNLSL PGAIGSSGSF SQRSHLPSSG TSTPSSVVDI PPPFDLACIT KKPITKSSPS LLIDGDTLEK ASKKKKSSFK RFLELTFRKK TESKVHVDMN LSSSRSSSES SYHGPARVLE LDRRSLSNSP QLKCRTGKLR ASDSPAALIF YRDSKRKGVP FSRTVSRVES FEDRSRPPFL PLPLTKPRSI SFPNADTSDY ENIPAMNSDY ENIQIPPRRP VRTGTFTKLF EEQSRALSTA NENDGYVDMS SFNAFESKQQ SSEQEAESAY TEPYKVCPIS AAPREDLTSD EEQGSSEEED SASRDPSLSH KGEGQSRALV IAQELLSSEK AYVQMLQHLS LDFHGAVLRA LENVEQEGRE PLAQEELRQG LRELPAICDL HQGILESLEQ RLGDCGEGQP QVADIFLARE QEFEHHAAHI LQFDRYLGLL AESCLLSPRL ATTVREFEQS SQGGGQSMKH RMLRVVQRLF QYQVLLTDYL NNLCPDSAEY DNTQSALTLI SKVTDRANES MEQGENLQKL VHIEYSVRGQ GDLLQPGREF LKEGTLMRVR GKSRHPRHLF LMNDTLLYTH PQKDGKYRLK SSLPVANMKV SRPVMDKVPY ALKIETPESC LTLSASSCAE RDEWHYCLSR ALPEDYKTQA LAAFHHSVEI RERLGISLGE RLPTLVPVTH AMMCMNCGCD FSLTVRRHHC HACGKIVCRN CSRNKYPLKC LKNRMAKVCD GCFRELKLRN GPVPGSMRER PVSMSFPLSS SRFSSGSALS SVFQSISPST FKKQKKVPSA LSEVAASGEG SAISGYLSRC KSGKRRWKKL WLVIKGKVLY TYLASEDKVA MESIPLLGFT IAPEKEEGSS EVGPVFHLYH KKTLFYSFKA EDSNSAQRWM EAMEDASVL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: FGD5 (FYVE, RhoGEF and PH Domain Containing 5 (FGD5))

Andere Bezeichnung: Fgd5 (FGD5 Produkte)

Synonyme: ZFYVE23 Protein, C330025N11Rik Protein, mFLJ00274 Protein, FYVE, RhoGEF and PH domain containing 5 Protein, Fgd5 Protein, FGD5 Protein

Hintergrund: FYVE, RhoGEF and PH domain-containing protein 5,FUNCTION: Activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. Mediates VEGF-induced CDC42 activation. May regulate proangiogenic action of VEGF in vascular endothelial cells, including network formation, directional movement and proliferation. May play a role in regulating the actin cytoskeleton and cell shape (By similarity). {ECO:0000250}.

Molekulargewicht: 134.7 kDa

UniProt: Q80UZ0