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Nischarin Protein (NISCH) (AA 1-1593) (His tag)

Crystallography grade NISCH Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3135991
  • Target Alle Nischarin (NISCH) Proteine anzeigen
    Nischarin (NISCH)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1593
    Spezies
    • 4
    • 1
    Maus
    Quelle
    • 2
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Nischarin Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MAAATLSFGP EREAEPAKEA RVVGSELVDT YTVYVIQVTD GNHEWTIKHR YSDFHDLHEK LVAERKIDKS LLPPKKIIGK NSRSLVEKRE RDLEVYLQTL LTTFPDVAPR VLAHFLHFHL YEVNGVTAAL AEELFEKGEQ LLGAGEVFAI RPLQLYAITE QLQQGKPTCA SGDAKTDLGH ILDFTCRLKY LKVSGTEGPF GTSNIKEQLL PFDLSIFKSL HQVEISHCDA KHIRGLVTSK PTLATMSVRF SATSMKEVLA PEASEFDEWE PEGTATLGGP VTAIIPTWQA LTTLDLSHNS ICEIDESVKL IPKIEYLDLS HNGLRVVDNL QHLYNLVHLD LSYNKLSSLE GVHTKLGNVK TLNLAGNFLE SLSGLHKLYS LVNVDLRDNR IEQLDEVKSI GSLPCLERLT LLNNPLSIIP DYRTKVLSQF GERASEICLD DVATTEKELD TVEVLKAIQK AKDVKSKLSN TEKKAGEDFR LPPAPCIRPG GSPPAAPASA SLPQPILSNQ GIMFVQEEAL ASSLSSTDSL PPEDHRPIAR ACSDSLESIP AGQVASDDLR DVPGAVGGVS PDHAEPEVQV VPGSGQIIFL PFTCIGYTAT NQDFIQRLST LIRQAIERQL PAWIEAANQR EEAHGEQGEE EEEEEEEEDV AENRYFEMGP PDAEEEEGSG QGEEDEEDED EEAEEERLAL EWALGADEDF LLEHIRILKV LWCFLIHVQG SIRQFAACLV LTDFGIAVFE IPHQESRGSS QHILSSLRFV FCFPHGDLTE FGFLMPELCL VLKVRHSENT LFIISDAANL HEFHADLRSC FAPQHMAMLC SPILYGSHTT LQEFLRQLLT FYKVAGGSQE RSQGCFPVYL VYSDKRMVQT PAGDYSGNIE WASCTLCSAV RRSCCAPSEA VKSAAIPYWL LLTSQHLNVI KADFNPMPNR GTHNCRNRNS FKLSRVPLST VLLDPTRSCT QPRGAFADGH VLELLVGYRF VTAIFVLPHE KFHFLRVYNQ LRASLQDLKT VVISKNPSAK PRNQPAKSRA SAEQRLQETP ADAPAPAAVP PTASAPAPAE ALAPDLAPVQ APGEDRGLTS AEAPAAAEAP AAAEAPAAAE APAAAEAPAA AEAPAAAEAP APAEAPAAAE APAAAEAPAA AEAPAAAEAP ASAEAPAPNQ APAPARGPAP ARGPAPAGGP APAEALAQAE VPAQYPSERL IQSTSEENQI PSHLPVCPSL QHIARLRGRA IIDLFHNSIA EVENEELRHL LWSSVVFYQT PGLEVTACVL LSSKAVYFIL HDGLRRYFSE PLQDFWHQKN TDYNNSPFHV SQCFVLKLSD LQSVNVGLFD QYFRLTGSSP TQVVTCLTRD SYLTHCFLQH LMLVLSSLER TPSPEPVDKD FYSEFGDKNT GKMENYELIH SSRVKFTYPS EEEVGDLTYI VAQKMADPAK NPALSILLYI QAFQVVTPHL GRGRGPLRPK TLLLTSAEIF LLDEDYIHYP LPEFAKEPPQ RDRYRLDDGR RVRDLDRVLM GYYPYPQALT LVFDDTQGHD LMGSVTLDHF GEMPGGPGRV GQGREVQWQV FVPSAESREK LISLLARQWE ALCGRELPVE LTG
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Nisch Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product NISCH Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Nischarin (NISCH)
    Andere Bezeichnung
    Nisch (NISCH Produkte)
    Synonyme
    NISCH Protein, IRAS Protein, 1200007D05Rik Protein, 3202002H23Rik Protein, AW494485 Protein, I-1 Protein, IR1 Protein, hIRAS Protein, nischarin Protein, nischarin S homeolog Protein, NISCH Protein, Cyan7822_6841 Protein, nisch.S Protein, nisch Protein, Nisch Protein
    Hintergrund
    Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways. Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity. Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation. Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation. Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells. Inhibits lamellipodia formation, when overexpressed. Plays a role in protection against apoptosis (By similarity). Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3 (By similarity). When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures (By similarity). {ECO:0000250, ECO:0000269|PubMed:11121431, ECO:0000269|PubMed:12915132, ECO:0000269|PubMed:15229651, ECO:0000269|PubMed:16002401, ECO:0000269|PubMed:16678176, ECO:0000269|PubMed:18332102}.
    Molekulargewicht
    176.0 kDa Including tag.
    UniProt
    Q80TM9
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