MYBBP1A Protein (AA 2-1344) (His tag)

Produktnummer ABIN3135937

Produktdetails

Target: MYBBP1A (Myb-Binding Protein 1A (MYBBP1A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-1344

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses MYBBP1A Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: AEMKSPTKAE PATPAEAAQS DRHSLLEHSR EFLDFFWDIA KPDQETRLRA TEKLLEYLRT RPNDSEMKYA LKRLITGLGV GREAARPCYS LALAQLLQSF EDIPLCDILD QIQEKYSLQA MNKAMMRPSL FANLFGVLAL FQSGRLVKDK EALMKSVQLL KILSQHPNHL QGQPIKALVD ILSEVPESMF QEILPKVLKG NMKVILRSPK YLELFLLAKQ RVPTKLESLM GSVDLFSEDN IPSLVNILKV AANSVKKEHK LPNVALDLLR LALKESRFEL FWKKVLEEGL LKNPSWTSSY MCFRLLGASL PLLSEEQLQL VMRGDLIRHF GENMVISKPQ NLFKIIPEIS TYVGTFLEGC QDDPKRQLTM MVAFTTITNQ GLPVMPTFWR VTRFLNAEAL QSYVAWLRDM FLQPDLNSLV DFSTANQKRA QDASLNVPER AVFRLRKWII HRLVSLVDHL HLEKDEAVVE QIARFCLFHA FFKTKKATPQ IPETKQHFSF PLDDRNRGVF VSAFFSLLQT LSVKFRQTPD LAENGKPWTY RLVQLADMLL NHNRNVTSVT SLTTQQRQAW DQMMSTLKEL EARSSETRAI AFQHLLLLVG LHIFKSPAES CDVLGDIQTC IKKSMEQNPR RSRSRAKASQ EPVWVEVMVE ILLSLLAQPS NLMRQVVRSV FGHICPHLTP RCLQLILAVL SPVTNEDEDD NVVVTDDADE KQLQHGEDED SDNEDNKNSE SDMDSEDGEE SEEEDRDKDV DPGFRQQLME VLKAGNALGG VDNEEEEELG DEAMMALDQN LASLFKEQKM RIQARNEEKN KLQKEKKLRR DFQIRALDLI EVLVTKQPEH PLILELLEPL LNVIQHSMRS KGSTKQEQDL LHKTARIFMH HLCRARRYCH EVGPCAEALH AQVERLVQQA GSQADASVAL YYFNASLYLL RVLKGNTNKR HQDGHKLHGA DTEDSEDQAA NCLDLDFVTR VYSASLESLL TKRNSSLTVP MFLSLFSRYP VICKNLLPVL AQHVAGPSRP RHQAQACLML QKTLSARELR VCFEDPEWEQ LITQLLGKAT QTLQTLGEAQ SKGEHQKELS ILELLNTLLR TVNHEKLSVD LTAPLGVLQS KQQKLQQSLQ QGNHSSGSNR LYDLYWQAMR MLGVQRPKSE KKNAKDIPSD TQSPVSTKRK KKGFLPETKK RKKLKSEGTT PEKNAASQQD AVTEGAMPAA TGKDQPPSTG KKKRKRVKAS TPSQVNGITG AKSPAPSNPT LSPSTPAKTP KLQKKKEKLS QVNGATPVSP IEPESKKHHQ EALSTKEVIR KSPHPQSALP KKRARLSLVS RSPSLLQSGV KKRRVASRRV QTP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Mybbp1a Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MYBBP1A (Myb-Binding Protein 1A (MYBBP1A))

Andere Bezeichnung: Mybbp1a (MYBBP1A Produkte)

Synonyme: MYBBP1A Protein, MGC98482 Protein, p160 Protein, pap2 Protein, AL024407 Protein, AU019902 Protein, P160 Protein, p160MBP Protein, p67MBP Protein, DBP Protein, PAP2 Protein, cb486 Protein, sb:cb486 Protein, MYB binding protein 1a Protein, MYB binding protein (P160) 1a S homeolog Protein, myb-binding protein 1A Protein, MYB binding protein (P160) 1a Protein, MYBBP1A Protein, mybbp1a.S Protein, mybbp1a Protein, LOC100413205 Protein, Mybbp1a Protein

Hintergrund: May activate or repress transcription via interactions with sequence specific DNA-binding proteins. Repression may be mediated at least in part by histone deacetylase activity (HDAC activity). Acts as a corepressor and in concert with CRY1, represses the transcription of the core circadian clock component PER2. Preferentially binds to dimethylated histone H3 'Lys-9' (H3K9me2) on the PER2 promoter (PubMed:19129230). {ECO:0000269|PubMed:11956195, ECO:0000269|PubMed:14744933, ECO:0000269|PubMed:19129230}.

Molekulargewicht: 152.9 kDa Including tag.

UniProt: Q7TPV4