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DZIP3 Protein (AA 1-1204) (His tag)

Crystallography grade DZIP3 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3135936
  • Target Alle DZIP3 Proteine anzeigen
    DZIP3 (DAZ Interacting Protein 3, Zinc Finger (DZIP3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1204
    Spezies
    Maus
    Quelle
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses DZIP3 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MDSLAEEFFV SGNPDVEEQT KEETEIIAEK PVTQLDKQKM DISADPEPVN ALLEIKKVLN PISALPKGVF PNIEKFIQED FSFQTMQREV TTHSQTGEEI VPALTLHFLI TQLEMALRNI QASNYTAQQI NVGYYLTLLF LYGVALTERA KKEDCIEAEN KFLVMKMVIQ ESEICENFMC LVYFGRGLLR CAQKRYNGAL LEFYKSLQEI GDTDDNWFEV DPTDDEDLPT TFKDSLNNFI KTTESNIMKE TICSYLDCER SCEADILKNT NYKGFFQLMC SKSCCIYFHK ICWKKFKNLK YPGESDQSFS GQKCLKEGCP GDMVRMLQCD VPGIVKILFE VVRKDEYITI ENLGASYKNL MSLELTDTDI RPKFNLKPNT KDEVPIFKLD YNYFYHLLHI IIISGTDMVR QIFDEAMPPT LLKKELLIHK NVLEPYYNHL WTNHPLGGSW HLLYPPNKEL PQSKQFDLCL LLALIKHLNV FPAPRKGWDM EPPSSDLSKS ADILRLCKYR DILLSEILMN GLTELQFNSI WKKVSDILLR LGMKQDDLDK VKENPIENIS LDYHQLSIYL GIPVPEIIQR MLSCYQQGIT LQSITGSQRL DVEEFQNDEE DLSPPVMEYN IDVKSNTEIQ LAEINKDVAS IPSESSTESV KDLQEVKSKT KKKKRTKSNK KDKDSEDEQV SYMVEKDDQL ETEQVDVNTL STYMKTDTSD AQEDSAAEDK FCSLDELHIL DMVEQGSSGK ESTDFKETEK ERLAHQHQLY KLQYECEDYK RQLKTVTFRW QENQMLIKKK EKIIVSLNQQ VAFGINKMSK LQRQIHAKDD EIKNLKDQLS LKRSQWEMEK HNLESTVKTY LNKLNAETSR ALTAEVYFLQ CRRDFGLLHL EQTEKECLNQ LARVTHMAAS NLESLQLKAA VDSWNAIVAD VRNKIAFLRT QYNEQINKVK QGFALSTLPP VQLPPPPPSP EILIQQFLGR PLVKESFFRP ILTVPQMPAV CPGVISAAVQ PRPPLMPGIT WAMPTPIGDT VSPSASLCSE PLMINWERIT DRLKTAFPQQ TRKELTDFLQ QLKDSHGKSV SRLTFDEIVY KISQMIEPKK SESEEKSAQD GNNASPSHTA SQPNAPQDPK SAQGSATWEG DKDMDNEEEE EEPCVICHEN LSPENLSVLP CAHKFHSQCI RPWLMQQGTC PTCRLHVLQP EEFPGHPNGQ LPKI
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Dzip3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product DZIP3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    DZIP3 (DAZ Interacting Protein 3, Zinc Finger (DZIP3))
    Andere Bezeichnung
    Dzip3 (DZIP3 Produkte)
    Synonyme
    2310047C04Rik Protein, 2A-HUB Protein, 6430549P11Rik Protein, A230104G20 Protein, PPP1R66 Protein, UURF2 Protein, hRUL138 Protein, RGD1563278 Protein, DAZ interacting protein 3, zinc finger Protein, DAZ interacting zinc finger protein 3 Protein, Dzip3 Protein, DZIP3 Protein
    Hintergrund
    E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA (By similarity). {ECO:0000250}.
    Molekulargewicht
    139.0 kDa Including tag.
    UniProt
    Q7TPV2
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