Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

MAML1 Protein (AA 1-1020) (His tag)

Crystallography grade MAML1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3135840
  • Target Alle MAML1 Proteine anzeigen
    MAML1 (Mastermind-Like 1 (MAML1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1020
    Spezies
    Maus
    Quelle
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses MAML1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MVLPTCPMAE FALPRHSAVM ERLRRRIELC RRHHSTCEAR YEAVSPERLE LERQHTFALH QRCIQAKAKR AGKHRQPPAA ATAPVAAPAP ASAPAAARLD AADGPEHGRP VAHLHDTVKR SLDSAASPQN GDQPNGYGDL FPGHKKTRRE APLGVSVSAN GLPPASPLGQ PDKPSGGDTL QTAGKHSLGL DPINKKCLAD SGIHLNGGSN SSEPFPLSLS KELKQEPVDD LPCMIAGAGG SVAQSNLMPD LNLNEQEWKE LIEELNRSVP DEDMKDLFTE DFEEKKDPEP PGSATQTPLA QDINIKTEFS PAAFEQEQLG SPQVRAGSAG QTFLGASSAP VGTDSPSLGS SQTLFHTTSQ PGVDNSSPNL MPASAQAQSA QRALTSVVLP SQGPGGASEL SSAHQLQQIA AKQKREQMLQ NPQQAAPAPG PGQLATWQQA GPSHSPLDVP YPMEKPASPS GYKQDFTNSK LLMMPGVNKS SPRPGGPYLQ PSHSNLLSHQ SPSNLNQNPV NNQGSVLDYG NTKPLSHYKA DCGQGGPGSG QNKPALMAYL PQQLPHLSNE QNSLFLMKPK SGNMPFRSLV PPGQEQNPSS VPVAAPAASV GTQPTVSVAS THNSSPYLSS QQQAAVMKQH QLLLDQQKQR EQQQQQLQQQ QFLQRQHLLA EQEKQQFQRH LTRPPPQYQD PTQSTFPQQV GQFTGPSAAV PGMNNLGPSN SSCPRVFPQP GTLMSMGPGH APVSSLPSSS GQQDRGVAQF TGSQSLPQNS LYGMASGLAQ IVAQPPPQAT STHAHIPRQT NVGQNASTSA AYGQNSLGSA SLSQQHSKGT LPPGLTKPQV PRVSAAMGSQ NASWQHQGMP NLSSQTSGNS SVNPFTAAPS FHIQQAHLKL AGQQFSQAMP SRPMAPLSSA GAAGPMLPPV SAQQRNSAPA SAPPQAAPQQ GLPGLSPSGP ELGAFGQSPT SQMSGRPGLH CAQAYPVRTM GQELPFAYSG QPGSSGLSSV AGHTDLIDSL LKNRTSEEWI NELDDLLGSQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Maml1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MAML1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    MAML1 (Mastermind-Like 1 (MAML1))
    Andere Bezeichnung
    Maml1 (MAML1 Produkte)
    Synonyme
    mam1 Protein, mam-1 Protein, xmam1 Protein, MAML1 Protein, AI644666 Protein, AW743257 Protein, D930008C07Rik Protein, Mam-1 Protein, Mam1 Protein, mKIAA0200 Protein, mastermind like transcriptional coactivator 1 L homeolog Protein, mastermind like transcriptional coactivator 1 Protein, mastermind like 1 (Drosophila) Protein, mastermind-like transcriptional coactivator 1 Protein, maml1.L Protein, MAML1 Protein, Maml1 Protein
    Hintergrund
    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. {ECO:0000269|PubMed:15019995, ECO:0000269|PubMed:15187027}.
    Molekulargewicht
    108.7 kDa Including tag.
    UniProt
    Q6T264
    Pathways
    Notch Signalweg, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
Sie sind hier:
Kundenservice