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NFRKB Protein (AA 1-1296) (His tag)

Crystallography grade NFRKB Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3135824
  • Target Alle NFRKB Proteine anzeigen
    NFRKB (Nuclear Factor Related To KappaB Binding Protein (NFRKB))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1296
    Spezies
    Maus
    Quelle
    • 2
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses NFRKB Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MDSLDHMLTD PLELGPCGDG HSTGIMEDCL LGGTRVSLPE DLLEDPEIFF DVVSLSTWQE VLSDSQREHL QQFLPRFPAD SVEQQRELIL ALFSGENFRF GNPLHIAQKL FRDGHFNPEV VKYRQLCFKS QYKRYLNSQQ QYFHRLLKQI LASRSDLLEM ARRSGPALPF PHKHHSPSRS PEEREWRTQQ RYLKVLREVK EECGDTALSS DEEDLSSWLP SSPARSPSPA VPLRVVPTLS TTDMKTADKI ELGDSDLKLM LKKHHEKRKH QPDHPDLLTG DLTLSDIMTR VNAGRKGSLA ALYDLAVLKK KVKEKEEKKK KKIKLIKSEA EDLAEPLSST EGVPTLSQAP SPLAISSIKE EPLEDIKPCL GINEISSSFF SLLLEILLLE SQASLPMLED RVLDWQSSPA SSLNSWFSAA PNWAELVLPA LQYLAGESRA VPSSFSPFVE FKEKTQQWKL LGQSQDNEKE LAALFHLWLE TKDQAFCKEN EDSSDAMTPV PRVRTDYVVR PSTGEEKRVF QEQERYRYSQ PHKAFTFRMH GFESVVGPVK GVFDKETSLN KAREHSLLRS DRPAYVTILS LVRDAAARLP NGEGTRAEIC ELLKDSQFLA PDVTSTQVNT VVSGALDRLH YEKDPCVKYD IGRKLWIYLH RDRSEEEFER IHQAQAAAAK ARKALQQKPK PPSKVKSSNK EGSTKGLSGP SEQSQMSLSD SSMPPTPVTP VTPTTPALPT PISPPPVSAV NRSGSSTVSE PAQSSSGVLL VSSPTMPQLG TMLSPASIQT PPSSQATARV VSHSSSAGLP QVRVVAQPSL PAVSQQSVGP AQPLPQMPAG PQIRVPVTAT QTKVVPQAVM ATVPVKGQTA AASVQRPGPG QTGLTVTNLP AAVSPVSKTA MSSPGNSAPS ASTTAVIQNV TGQNIIKQVS ITGQLGVKPQ TGSSIPLTAT NFRIQGKDVL RLPPSSITTD AKGQTVLRIT PDMMATLAKS QVTTVKLTQD LFGAGSGTAG KGISATLHVT SNPVHAADSP AKAPSASVPS SAPAGTTVVK VTPDLKPTET ANSAFRLMPA LGVSVADQKG KNTVASSEAK PAATIRIVQG LGVMPPKAGQ TITVAAHAKQ GASVAGGSGT VHSSTVSLPS INAAVSKTVA VASGATSTPI SIGTGAPTVR QVPVNTTVVS TSQSGKLPTR ITVPLSVISQ PMKGKSVVTA PIIKGNLGAN LSGLGRNIIL TTMPAGTKLI AGNKPVSFLT AQQLQQLQQQ GQATQVRIQT VPASHLQQGT ASGSSKAVST VVVTTAPSPK QAPEQQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Nfrkb Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product NFRKB Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    NFRKB (Nuclear Factor Related To KappaB Binding Protein (NFRKB))
    Andere Bezeichnung
    Nfrkb (NFRKB Produkte)
    Synonyme
    nfrkb Protein, MGC75871 Protein, NFRKB Protein, A530090G11Rik Protein, INO80G Protein, nuclear factor related to kappaB binding protein Protein, amyloid beta precursor like protein 2 Protein, nuclear factor related to kappa B binding protein Protein, nfrkb Protein, NFRKB Protein, APLP2 Protein, Nfrkb Protein
    Hintergrund
    Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'. {ECO:0000250}., Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Modulates the deubiquitinase activity of UCHL5 in the INO80 complex (By similarity). {ECO:0000250}.
    Molekulargewicht
    139.7 kDa Including tag.
    UniProt
    Q6PIJ4
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