DDX11 Protein (AA 1-880) (His tag)

Produktnummer ABIN3135677

Produktdetails

Target: DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-880

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses DDX11 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: MADENQEIGG IHFPFPFPPY PIQKDFMAEL YKVLEGGKIG IFESPTGTGK SLSLICGALS WLRDFEKKKL QAEALLLAPG SGPPSSEKNS LLTSSSCQEP TDTPRPAGEP DWVTEFVQKK EERDLVERLR EEQKHEEEET EALLRLSREM LDAGTGPEQL EQLECGEEHL VLAEYESDEE RRGSRVDEAE DDLEEEHITK IYYCSRTHSQ LAQFVREVLK SPFGKETRLV SLGSRQTLCV NEDVKNLGSV QLMNDRCVDM QRSKREKNGT GEDKPKRKRQ KIQTSCPFYN HEQMELLRDE ILLEVKDMEQ LVALGKEARA CPYYGSRFAI PAAQLVVLPY PMLLHAATRQ AAGIRLQGQV VIIDEAHNLI DTITNIHSTE VNGSQLCQAH SQLLQYMERY RKRLKAKNLM YIKQILYLLE KFVAVLGGNV KQNPTTQSLS QTGSELKSIN DFLFQSQVDN INLFKVQRYL EKSMLSRKLF GFTECFGVVL PSLSDSQENR GLAGFQQFLK SLQSGPTEDS PEEGQAVALR PASPLMHIEA FLAALTTANQ DGRVIVNRQG SVGQSSLKFL LLNPAVHFAQ VVKECRAVVI AGGTMQPMSD FREQLLACSG VEAGRVVEFS CGHVIPPDNI LPLIICSGPS NQQLEFTYQR RELPQMVEET GRILCNLCNV VPGGVVCFLP SYEYLRQVHA HWDKTGLLTR LSVRKKIFQE PKRASQVEQV LMAYSKCIMS CSHSEGHLTG ALLLSVVGGK MSEGINFSDD LGRCVVMVGM PYPNIKSPEL QEKMAYLNQT LPRTQGQPLP GTVLIENLCM KAINQSIGRA IRHQRDFASI VLLDHRYARP SILAKLPAWI RDRVEVKATF GPAFAAVRKF HREKSHPSLV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ddx11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: DDX11 (DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 11 (DDX11))

Andere Bezeichnung: Ddx11 (DDX11 Produkte)

Synonyme: CHL1 Protein, CHLR1 Protein, KRG2 Protein, WABS Protein, 4732462I11Rik Protein, essa15a Protein, putative ATP-dependent RNA helicase DDX11-like protein 8 Protein, DEAD/H-box helicase 11 Protein, DEAD/H (Asp-Glu-Ala-Asp/His) box helicase 11 Protein, LOC100448472 Protein, DDX11 Protein, Ddx11 Protein

Hintergrund: DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA (By similarity). {ECO:0000250|UniProtKB:Q96FC9, ECO:0000269|PubMed:17611414}.

Molekulargewicht: 99.7 kDa Including tag.

UniProt: Q6AXC6

Pathways: ER-Nucleus Signaling