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Periostin Protein (POSTN) (AA 24-838) (His tag)

Crystallography grade POSTN Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3135487
  • Target Alle Periostin (POSTN) Proteine anzeigen
    Periostin (POSTN)
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 24-838
    Spezies
    • 12
    • 5
    • 3
    Maus
    Quelle
    • 10
    • 6
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses Periostin Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    NSYYDKVLAH SRIRGRDQGP NVCALQQILG TKKKYFSSCK NWYQGAICGK KTTVLYECCP GYMRMEGMKG CPAVMPIDHV YGTLGIVGAT TTQHYSDVSK LREEIEGKGS YTYFAPSNEA WENLDSDIRR GLENNVNVEL LNALHSHMVN KRMLTKDLKH GMVIPSMYNN LGLFINHYPN GVVTVNCARV IHGNQIATNG VVHVIDRVLT QIGTSIQDFL EAEDDLSSFR AAAITSDLLE SLGRDGHFTL FAPTNEAFEK LPRGVLERIM GDKVASEALM KYHILNTLQC SEAITGGAVF ETMEGNTIEI GCEGDSISIN GIKMVNKKDI VTKNGVIHLI DEVLIPDSAK QVIELAGKQQ TTFTDLVAQL GLASSLKPDG EYTLLAPVNN AFSDDTLSMD QRLLKLILQN HILKVKVGLS DLYNGQILET IGGKQLRVFV YRTAICIENS CMVRGSKQGR NGAIHIFREI IQPAEKSLHD KLRQDKRFSI FLSLLEAADL KDLLTQPGDW TLFAPTNDAF KGMTSEEREL LIGDKNALQN IILYHLTPGV YIGKGFEPGV TNILKTTQGS KIYLKGVNET LLVNELKSKE SDIMTTNGVI HVVDKLLYPA DIPVGNDQLL ELLNKLIKYI QIKFVRGSTF KEIPMTVYTT KIITKVVEPK IKVIQGSLQP IIKTEGPAMT KIQIEGDPDF RLIKEGETVT EVIHGEPVIK KYTKIIDGVP VEITEKQTRE ERIITGPEIK YTRISTGGGE TGETLQKFLQ KEVSKVTKFI EGGDGHLFED EEIKRLLQGD TPAKKIPANK RVQGPRRRSR EGRSQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Postn Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product POSTN Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    Periostin (POSTN)
    Andere Bezeichnung
    Postn (POSTN Produkte)
    Synonyme
    fa99h07 Protein, pn Protein, postn Protein, wu:fa99h07 Protein, wu:fc70f09 Protein, zgc:153873 Protein, LOC100008945 Protein, osf-2 Protein, pdlpostn Protein, periostin Protein, OSF-2 Protein, OSF2 Protein, PDLPOSTN Protein, PN Protein, RP11-412K4.1 Protein, A630052E07Rik Protein, AI747096 Protein, Osf2 Protein, PLF Protein, peri Protein, Plf Protein, periostin Protein, periostin, osteoblast specific factor b Protein, periostin, osteoblast specific factor Protein, POSTN Protein, postnb Protein, postn Protein, Postn Protein
    Hintergrund
    Induces cell attachment and spreading and plays a role in cell adhesion (PubMed:10404027). Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX (PubMed:20181949). {ECO:0000269|PubMed:10404027, ECO:0000269|PubMed:20181949}.
    Molekulargewicht
    91.6 kDa Including tag.
    UniProt
    Q62009
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