LTBP3 Protein (AA 39-1268) (His tag)

Produktnummer ABIN3135462

Produktdetails

Target: LTBP3 (Latent Transforming Growth Factor beta Binding Protein 3 (LTBP3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 39-1268

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses LTBP3 Protein ist gelabelt mit His tag.

Applikation: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: EGGPAGERGT GGGGALARER FKVVFAPVIC KRTCLKGQCR DSCQQGSNMT LIGENGHSTD TLTGSAFRVV VCPLPCMNGG QCSSRNQCLC PPDFTGRFCQ VPAAGTGAGT GSSGPGLART GAMSTGPLPP LAPEGESVAS KHAIYAVQVI ADPPGPGEGP PAQHAAFLVP LGPGQISAEV QAPPPVVNVR VHHPPEASVQ VHRIEGPNAE GPASSQHLLP HPKPPHPRPP TQKPLGRCFQ DTLPKQPCGS NPLPGLTKQE DCCGSIGTAW GQSKCHKCPQ LQYTGVQKPV PVRGEVGADC PQGYKRLNST HCQDINECAM PGNVCHGDCL NNPGSYRCVC PPGHSLGPLA AQCIADKPEE KSLCFRLVST EHQCQHPLTT RLTRQLCCCS VGKAWGARCQ RCPADGTAAF KEICPGKGYH ILTSHQTLTI QGESDFSLFL HPDGPPKPQQ LPESPSRAPP LEDTEEERGV TMDPPVSEER SVQQSHPTTT TSPPRPYPEL ISRPSPPTFH RFLPDLPPSR SAVEIAPTQV TETDECRLNQ NICGHGQCVP GPSDYSCHCN AGYRSHPQHR YCVDVNECEA EPCGPGKGIC MNTGGSYNCH CNRGYRLHVG AGGRSCVDLN ECAKPHLCGD GGFCINFPGH YKCNCYPGYR LKASRPPICE DIDECRDPST CPDGKCENKP GSFKCIACQP GYRSQGGGAC RDVNECSEGT PCSPGWCENL PGSYRCTCAQ YEPAQDGLSC IDVDECEAGK VCQDGICTNT PGSFQCQCLS GYHLSRDRSR CEDIDECDFP AACIGGDCIN TNGSYRCLCP LGHRLVGGRK CKKDIDECSQ DPGLCLPHAC ENLQGSYVCV CDEGFTLTQD QHGCEEVEQP HHKKECYLNF DDTVFCDSVL ATNVTQQECC CSLGAGWGDH CEIYPCPVYS SAEFHSLVPD GKRLHSGQQH CELCIPAHRD IDECILFGAE ICKEGKCVNT QPGYECYCKQ GFYYDGNLLE CVDVDECLDE SNCRNGVCEN TRGGYRCACT PPAEYSPAQA QCLIPERWST PQRDVKCAGA SEERTACVWG PWAGPALTFD DCCCRQPRLG TQCRPCPPRG TGSQCPTSQS ESNSFWDTSP LLLGKSPRDE DSSEEDSDEC RCVSGRCVPR PGGAVCECPG GFQLDASRAR CVDIDECREL NQRGLLCKSE RCVNTSGSFR CVCKAGFTRS RPHGPACLSA AADDAAIAHT SVIDHRGYFH
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ltbp3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: LTBP3 (Latent Transforming Growth Factor beta Binding Protein 3 (LTBP3))

Andere Bezeichnung: Ltbp3 (LTBP3 Produkte)

Synonyme: LTBP-3 Protein, LTBP2 Protein, STHAG6 Protein, pp6425 Protein, Ltbp2 Protein, mFLJ00070 Protein, si:ch211-247o20.1 Protein, latent transforming growth factor beta binding protein 3 Protein, LTBP3 Protein, Ltbp3 Protein, ltbp3 Protein

Hintergrund: May be involved in the assembly, secretion and targeting of TGF- beta1 to sites at which it is stored and/or activated. May play critical roles in controlling and directing the activity of TGF-beta1. May have a structural role in the extra cellular matrix (ECM).

Molekulargewicht: 133.2 kDa Including tag.

UniProt: Q61810

Pathways: Growth Factor Binding