CTCF Protein (AA 1-736) (Strep Tag)

Produktnummer ABIN3135365

Produktdetails

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-736

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CTCF Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MEGEAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMICHTLPL PEGFQVVKVG ANGEVETLEQ GELPPQEDSS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE EQQEGLLSEV NAEKVVGNMK PPKPTKIKKK GVKKTFQCEL CSYTCPRRSN LDRHMKSHTD ERPHKCHLCG RAFRTVTLLR NHLNTHTGTR PHKCPDCDMA FVTSGELVRH RRYKHTHEKP FKCSMCDYAS VEVSKLKRHI RSHTGERPFQ CSLCSYASRD TYKLKRHMRT HSGEKPYECY ICHARFTQSG TMKMHILQKH TENVAKFHCP HCDTVIARKS DLGVHLRKQH SYIEQGKKCR YCDAVFHERY ALIQHQKSHK NEKRFKCDQC DYACRQERHM IMHKRTHTGE KPYACSHCDK TFRQKQLLDM HFKRYHDPNF VPAAFVCSKC GKTFTRRNTM ARHADNCAGP DGVEGENGGE TKKSKRGRKR KMRSKKEDSS DSEENAEPDL DDNEEEEEPA VEIEPEPEPQ PQPPPPPQPV APAPPPAKKR RGRPPGRTNQ PKQNQPTAII QVEDQNTGAI ENIIVEVKKE PDAEPAEGEE EEAQAATTDA PNGDLTPEMI LSMMDR
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Andere Bezeichnung: Ctcf (CTCF Produkte)

Synonyme: im:7152666 Protein, CTCF Protein, xctcf Protein, AW108038 Protein, CCCTC-binding factor Protein, CCCTC-binding factor (zinc finger protein) Protein, CTCF Protein, ctcf Protein, Ctcf Protein

Hintergrund: Transcriptional repressor CTCF (11-zinc finger protein) (CCCTC-binding factor) (CTCFL paralog),FUNCTION: Chromatin binding factor that binds to DNA sequence specific sites and regulates the 3D structure of chromatin (PubMed:28388437). Binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures, such as the nuclear lamina (PubMed:28388437). Defines the boundaries between active and heterochromatic DNA via binding to chromatin insulators, thereby preventing interaction between promoter and nearby enhancers and silencers (PubMed:28388437). Plays a critical role in the epigenetic regulation (By similarity). Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus (PubMed:16614224). On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2 (PubMed:16614224). Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory (PubMed:16614224). Regulates asynchronous replication of IGF2/H19 (PubMed:17329968). Plays a critical role in gene silencing over considerable distances in the genome (PubMed:16951251). Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones (By similarity). Inversely, binding to target sites is prevented by CpG methylation (By similarity). Plays an important role in chromatin remodeling (By similarity). Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping (By similarity). Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription (PubMed:16951251). When bound to chromatin, it provides an anchor point for nucleosomes positioning (By similarity). Seems to be essential for homologous X-chromosome pairing (PubMed:17952071). May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation (PubMed:11743158, PubMed:17952071, PubMed:15669143). May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation (PubMed:11743158, PubMed:17952071, PubMed:15669143). Involved in sister chromatid cohesion (By similarity). Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites (By similarity). Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (By similarity). Acts as a transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene (By similarity). Also binds to the PLK and PIM1 promoters (By similarity). Acts as a transcriptional activator of APP (By similarity). Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression (By similarity). Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription (PubMed:18614575). Seems to act as tumor suppressor (By similarity). {ECO:0000250|UniProtKB:P49711, ECO:0000269|PubMed:11743158, ECO:0000269|PubMed:15669143, ECO:0000269|PubMed:16614224, ECO:0000269|PubMed:16951251, ECO:0000269|PubMed:17329968, ECO:0000269|PubMed:17952071, ECO:0000269|PubMed:18614575, ECO:0000269|PubMed:28388437}.

Molekulargewicht: 83.7 kDa

UniProt: Q61164