LY75/DEC-205 Protein (AA 28-1667) (His tag)

Produktnummer ABIN3135301

Produktdetails

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Protein-Typ: Recombinant

Proteineigenschaft: AA 28-1667

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses LY75/DEC-205 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: SESSGNDPFT IVHENTGKCI QPLSDWVVAQ DCSGTNNMLW KWVSQHRLFH LESQKCLGLD ITKATDNLRM FSCDSTVMLW WKCEHHSLYT AAQYRLALKD GYAVANTNTS DVWKKGGSEE NLCAQPYHEI YTRDGNSYGR PCEFPFLIGE TWYHDCIHDE DHSGPWCATT LSYEYDQKWG ICLLPESGCE GNWEKNEQIG SCYQFNNQEI LSWKEAYVSC QNQGADLLSI HSAAELAYIT GKEDIARLVW LGLNQLYSAR GWEWSDFRPL KFLNWDPGTP VAPVIGGSSC ARMDTESGLW QSVSCESQQP YVCKKPLNNT LELPDVWTYT DTHCHVGWLP NNGFCYLLAN ESSSWDAAHL KCKAFGADLI SMHSLADVEV VVTKLHNGDV KKEIWTGLKN TNSPALFQWS DGTEVTLTYW NENEPSVPFN KTPNCVSYLG KLGQWKVQSC EKKLRYVCKK KGEITKDAES DKLCPPDEGW KRHGETCYKI YEKEAPFGTN CNLTITSRFE QEFLNYMMKN YDKSLRKYFW TGLRDPDSRG EYSWAVAQGV KQAVTFSNWN FLEPASPGGC VAMSTGKTLG KWEVKNCRSF RALSICKKVS EPQEPEEAAP KPDDPCPEGW HTFPSSLSCY KVFHIERIVR KRNWEEAERF CQALGAHLPS FSRREEIKDF VHLLKDQFSG QRWLWIGLNK RSPDLQGSWQ WSDRTPVSAV MMEPEFQQDF DIRDCAAIKV LDVPWRRVWH LYEDKDYAYW KPFACDAKLE WVCQIPKGST PQMPDWYNPE RTGIHGPPVI IEGSEYWFVA DPHLNYEEAV LYCASNHSFL ATITSFTGLK AIKNKLANIS GEEQKWWVKT SENPIDRYFL GSRRRLWHHF PMTFGDECLH MSAKTWLVDL SKRADCNAKL PFICERYNVS SLEKYSPDPA AKVQCTEKWI PFQNKCFLKV NSGPVTFSQA SGICHSYGGT LPSVLSRGEQ DFIISLLPEM EASLWIGLRW TAYERINRWT DNRELTYSNF HPLLVGRRLS IPTNFFDDES HFHCALILNL KKSPLTGTWN FTSCSERHSL SLCQKYSETE DGQPWENTSK TVKYLNNLYK IISKPLTWHG ALKECMKEKM RLVSITDPYQ QAFLAVQATL RNSSFWIGLS SQDDELNFGW SDGKRLQFSN WAGSNEQLDD CVILDTDGFW KTADCDDNQP GAICYYPGNE TEEEVRALDT AKCPSPVQST PWIPFQNSCY NFMITNNRHK TVTPEEVQST CEKLHSKAHS LSIRNEEENT FVVEQLLYFN YIASWVMLGI TYENNSLMWF DKTALSYTHW RTGRPTVKNG KFLAGLSTDG FWDIQSFNVI EETLHFYQHS ISACKIEMVD YEDKHNGTLP QFIPYKDGVY SVIQKKVTWY EALNACSQSG GELASVHNPN GKLFLEDIVN RDGFPLWVGL SSHDGSESSF EWSDGRAFDY VPWQSLQSPG DCVVLYPKGI WRREKCLSVK DGAICYKPTK DKKLIFHVKS SKCPVAKRDG PQWVQYGGHC YASDQVLHSF SEAKQVCQEL DHSATVVTIA DENENKFVSR LMRENYNITM RVWLGLSQHS LDQSWSWLDG LDVTFVKWEN KTKDGDGKCS ILIASNETWR KVHCSRGYAR AVCKIPLSPD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ly75 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: LY75/DEC-205 (LY75) (Lymphocyte Antigen 75 (LY75))

Andere Bezeichnung: Ly75 (LY75 Produkte)

Synonyme: LY75 Protein, MGC140308 Protein, DEC205 Protein, im:7150926 Protein, si:dkey-78p20.2 Protein, LYT75 Protein, CD205 Protein, DEC-205 Protein, CLEC13B Protein, GP200-MR6 Protein, LY-75 Protein, RGD1565962 Protein, Ly-75 Protein, lymphocyte antigen 75 Protein, CD205 protein Protein, CD302 antigen Protein, LY75 Protein, Cyan7822_2917 Protein, LY5 Protein, ly75 Protein, cd205 Protein, LOC100380704 Protein, LOC100433861 Protein, LOC100483541 Protein, Ly75 Protein

Hintergrund: Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Causes reduced proliferation of B lymphocytes (By similarity). {ECO:0000250}.

Molekulargewicht: 189.1 kDa Including tag.

UniProt: Q60767