TTF2 Protein (AA 1-1138) (Strep Tag)

Produktnummer ABIN3135203

Produktdetails

Target: TTF2 (Transcription Termination Factor, RNA Polymerase II (TTF2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1138

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TTF2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MDLVKCPEHG DACFLKTGVR DGPNKGKSFY VCRTNTCGFV QATDIPVSHC LLHEEFVVEL QGLFLPQDKK EWRLFFRCAR TKAEGKQWCG NVPWRQDPNP KELSVTSKPQ QPSESQLHSP SQPRNPFRVL NKNQKTLERK QFVEEGERKT ADKKLRENNE QLLDQRKEQK PKSNSRMEKD PSSDLVATRQ SGGDREEQEK SKFQPKTKKA EGMASKQGHG EVLQGIPKGP HMSESESRGV PNKPETLREK ETQLLVPSVP GQNPESKVQK EGHVSREPLK NGEAPSAQVT QRGLAQGPLQ GPSKTWRPVP EAPAAPELCS GMAHHATSSS EDSEDDGVSS RPGSPLLFDS TVDSQKKGSL QHSDQSVQRQ MPAASGVSKK GDSSDPAAQR ANLTTQLKQK KGTLAAVNIQ ALPDKGEKLL KQIQALEDAL SALALSPEQG TKEKCSAQEP EQSNITKAAA APLHLVPPQP LPRPLIQPAS SLGLKAGRQE TPEGASQCSG GHMNQHHLYN VWKITSEAID ELHRSLKSCP GETAVAEDPA GLKVPLLLHQ KQALAWLLWR ESQKPQGGIL ADDMGLGKTL TMIALILTKK NQQKSKEKER SEPVTWLSKN DSSVFTSSGT LIVCPASLIH HWKNEVEKRV TSNRLRIYLY HGPNRSRHAK VLSTYDIVIT TYSLLAKEIP TTKQEGEVPG ANLSVEGTSA PLLQVVWARI ILDEAHNVKN PRVQTSIAVC KLQAQARWAV TGTPIQNNLL DMYSLMKFLR CSPFDEFSLW KSQVDNGSMK GGERLSILTK SLLLRRTKDQ LDSTGKPLVA LPARRCQLHR LKLSEDERAV YDIFLARSRS ALQSYLKRQE GRGSHHGRSP DNPFSRVAQE FGSSVSQGCP AADSQRPSTV HVLSQLLRLR QCCCHLSLLK SALDPTELES EGLVLSLEEQ LSALTLSKVD VSEPSPTVSL NGTCFKAELF DDTRRSTKVS SLLAELEAIQ KGPGSQKSVI VSQWTSMLQV VALHLKKNRL TYATIDGSVN PKQRMDLVEA FNHSQGPQVM LISLLAGGVG LNLTGGNHLF LLDMHWNPSL EDQACDRIYR VGQKKDVVIH RFVCEGTVEE KILQLQEKKK DLAKQVLSGS EGPVTKLTLA DLKILFGI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TTF2 (Transcription Termination Factor, RNA Polymerase II (TTF2))

Andere Bezeichnung: Ttf2 (TTF2 Produkte)

Synonyme: HuF2 Protein, 4632434F22Rik Protein, 8030447N19 Protein, AV218430 Protein, MGC81081 Protein, TTF2 Protein, transcription termination factor 2 Protein, transcription termination factor, RNA polymerase II Protein, transcription termination factor, RNA polymerase II S homeolog Protein, TTF2 Protein, Ttf2 Protein, ttf2.S Protein

Hintergrund: Transcription termination factor 2 (EC 3.6.4.-) (RNA polymerase II termination factor) (Transcription release factor 2),FUNCTION: DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing (By similarity). {ECO:0000250}.

Molekulargewicht: 125.5 kDa

UniProt: Q5NC05

Pathways: Thyroid Hormone Synthesis