Sorbs2 Protein (AA 1-1180) (Strep Tag)

Produktnummer ABIN3135094

Produktdetails

Target: Sorbs2 (Sorbin and SH3 Domain Containing 2 (Sorbs2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1180

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses Sorbs2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MNTDSGGCAR KRAAMSVTLT SVKRVQSSPN LLAAGRESQS PDSAWRSYND RNPETLNGDA TYSSLAAKGF RSVRPNLQDK RSPTQSQITI NGNSGGAVSP VSYYQRPFSP SAYSLPASLN SSIIMQHGRS LDSAETYSQH AQSLDGTMGS SIPLYRSSEE EKRVTVIKAP HYPGIGPVDE SGIPTAIRTT VDRPKDWYKT MFKQIHMVHK PGLYNSPYSA QSHPAAKTQT YRPLSKSHSD NGTDAFKEVP SPVPPPHVPP RPRDQSSTLK HDWDPPDRKV DTRKFRSEPR SIFEYEPGKS SILQHERPVS IYQSSIDRSL ERPSSSASMA GDFRKRRKSE PAVGPLRGLG DQSSSRTSPG RADLPGSSST FTKSFISSSP SSPSRAQGGD DSKMCPPLCS YSGLNGTPSG ELECCNAYRQ HLDVPGDSQR AITFKNGWQM ARQNAEIWSS TEETVSPKIK SRSCDDLLND DCDSFPDPKT KSESMGSLLC EEDSKESCPM TWASPYIQEV CGNSRSRLKH RSAHNAPGFL KMYKKMHRIN RKDLMNSEVI CSVKSRILQY EKEQQHRGLL HGWSQSSTEE VPRDVVPTRI SEFEKLIQKS KSMPNLGDEM LSPITLEPPQ NGLCPKRRFS IESLLEEETQ VRHPSQGQRS CKSNTLVPIH IEVTSDEQPR THMEFSDSDQ DGVVSDHSDY VHVEGSSFCS ESDFDHFSFT SSESFYGSSH HHHHHHHHHR HLISSCKGRC PASYTRFTTM LKHERAKHEN MDRPRRQEMD PGLSKLAFLV SPVPFRRKKI LTPQKQTEKA KCKASVVEAL DSALKDICDQ IKAEKRRGSL PDNSILHRLI SELLPQIPER NSSLHALKRS PMHQPFHPLP PDGASHCPLY QNDCGRMPHS ASFPDVDTTS NYHAQDYGSA LSLQDHESPR SYSSTLTDLG RSASRERRGT PEKEKLPAKA VYDFKAQTSK ELSFKKGDTV YILRKIDQNW YEGEHHGRVG IFPISYVEKL TPPEKAQPAR PPPPVQPGEI GEAIAKYNFN ADTNVELSLR KGDRIILLKR VDQNWYEGKI PGTNRQGIFP VSYVEVVKRN AKGAEDYPDP PLPHSYSSDR IYTLSSNKPQ RPGFSHENIQ GGGEPFQALY NYTPRNEDEL ELRESDVVDV MEKCDDGWFV GTSRRTKFFG TFPGNYVKRL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Sorbs2 (Sorbin and SH3 Domain Containing 2 (Sorbs2))

Andere Bezeichnung: Sorbs2 (Sorbs2 Produkte)

Synonyme: Argbp2 Protein, 2010203O03Rik Protein, 9430041O17Rik Protein, A530071H08 Protein, mKIAA0777 Protein, argbp2 Protein, pro0618 Protein, 9430041O17RIK Protein, DKFZp468F1119 Protein, ARGBP2 Protein, PRO0618 Protein, sorbin and SH3 domain containing 2 Protein, Sorbs2 Protein, SORBS2 Protein, sorbs2 Protein

Hintergrund: Sorbin and SH3 domain-containing protein 2 (Arg-binding protein 2) (ArgBP2) (Arg/Abl-interacting protein 2),FUNCTION: Adapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 (By similarity). May play a role in the regulation of pancreatic cell adhesion, possibly by acting on WASF1 phosphorylation, enhancing phosphorylation by ABL1, as well as dephosphorylation by PTPN12 (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:O94875}.

Molekulargewicht: 132.3 kDa

UniProt: Q3UTJ2