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NLRC4 Protein (AA 1-1024) (His tag)

Crystallography grade NLRC4 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3135072
  • Target Alle NLRC4 Proteine anzeigen
    NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-1024
    Spezies
    Maus
    Quelle
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses NLRC4 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MNFIRNNRRA LIQRMGLTVT KQICDDLFAL NVLNNQEANV IYCEPLEQEA ARKIIHMTMQ KGSAACNLFL KSLENWDYFV YQDLTGQNLS YQVTEEDLNV LAQNLKDLYN SPAFLNFYPL GEDIDIIFNL EKTFTEPIMW KKDHRHHRVE QLTLGSLLEA LKSPCLIEGE SGKGKSTLLQ RIAMLWASGG CRALKGFRLV FFIHLRSARG GLFETLYDQL LNIPDFISKP TFKALLLKLH KEVLFLLDGY NEFHPQNCPE IEALIKENHR FKNMVIVTTT TECLRHIRHV GALTAEVGDM TEDSAKDLIE AVLVPDQVER LWAQIQESRC LRNLMKTPLF VVITCAIQMG RQEFQAHTQT MLFQTFYDLL IQKNSHRYRG GASGDFARSL DYCGDLALEG VFAHKFDFEP EHGSSMNEDV LVTIGLLCKY TAQRLKPTYK FFHKSFQEYT AGRRLSSLLT SKEPEEVSKG NSYLNKMVSI SDITSLYGNL LLYTCGSSTE ATRAVMRHLA MVYQHGSLQG LSVTKRPLWR QESIQSLRNT TEQDVLKAIN VNSFVECGIN LFSESMSKSD LSQEFEAFFQ GKSLYINSEN IPDYLFDFFE YLPNCASALD FVKLDFYERA TESQDKAEEN VPGVHTEGPS ETYIPPRAVS LFFNWKQEFK TLEVTLRDIN KLNKQDIKYL GKIFSSATNL RLHIKRCAAM AGRLSSVLRT CKNMHTLMVE ASPLTTDDEQ YITSVTGLQN LSIHRLHTQQ LPGGLIDSLG NLKNLERLIL DDIRMNEEDA KNLAEGLRSL KKMRLLHLTH LSDIGEGMDY IVKSLSEESC DLQEMKLVAC CLTANSVKVL AQNLHNLIKL SILDISENYL EKDGNEALQE LIGRLGVLGE LTTLMLPWCW DVHTSLPKLL KQLEGTPGLA KLGLKNWRLR DEEIKSLGEF LEMNPLRDLQ QLDLAGHCVS SDGWLYFMNV FENLKQLVFF DFSTEEFLPD AALVRKLSQV LSKLTLLQEV KLTGWEFDDY DISAIKGTFK LVTA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Nlrc4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product NLRC4 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    NLRC4 (NLR Family, CARD Domain Containing 4 (NLRC4))
    Andere Bezeichnung
    Nlrc4 (NLRC4 Produkte)
    Synonyme
    CARD12 Protein, CLAN Protein, CLAN1 Protein, CLANA Protein, CLANB Protein, CLANC Protein, CLAND Protein, CLR2.1 Protein, IPAF Protein, 9530011P19Rik Protein, Card12 Protein, NLR family CARD domain containing 4 Protein, NLR family, CARD domain containing 4 Protein, NLRC4 Protein, Nlrc4 Protein
    Hintergrund
    Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis. The NLRC4 inflammasome is activated as part of the innate immune response to a range of intracellular bacteria. It senses pathogenic proteins of the type III secretion system (T3SS) and type IV secretion system (T4SS) such as flagellin and PrgJ-like rod proteins via the Naip proteins (Naip1, Naip2 or Naip5): specific Naip proteins recognize and bind pathogenic proteins, driving assembly and activation of the NLRC4 inflammasome. The NLRC4 inflammasome senses Gram-negative bacteria such as L.pneumophila and P.aeruginosa, enteric pathogens S.typhimurium (Salmonella) and S.flexneri and fungal pathogen C.albicans. In intestine, the NLRC4 inflammasome is able to discriminate between commensal and pathogenic bacteria and specifically drives production of interleukin-1 beta (IL1B) in response to infection by Salmonella or P.aeruginosa. In case of L.pneumophila infection the inflammasome acts by activating caspase-7. {ECO:0000269|PubMed:15190255, ECO:0000269|PubMed:16648852, ECO:0000269|PubMed:16648853, ECO:0000269|PubMed:18070936, ECO:0000269|PubMed:19343209, ECO:0000269|PubMed:20133635, ECO:0000269|PubMed:20603313, ECO:0000269|PubMed:21874021, ECO:0000269|PubMed:21918512, ECO:0000269|PubMed:22174673, ECO:0000269|PubMed:22231517, ECO:0000269|PubMed:22484733, ECO:0000269|PubMed:22547706, ECO:0000269|PubMed:22885697}.
    Molekulargewicht
    117.7 kDa Including tag.
    UniProt
    Q3UP24
    Pathways
    Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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