TAX1BP1 Protein (AA 1-814) (Strep Tag)

Produktnummer ABIN3135062

Produktdetails

Target: TAX1BP1 (Tax1 (Human T-Cell Leukemia Virus Type I) Binding Protein 1 (TAX1BP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-814

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TAX1BP1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MTSFQEVQLQ TSNFAHVIFQ NVAKSYLPNA HLECHYTLTP YIHPHSKDWV GIFKVGWSTA RDYYTFLWSP MPEHYVEGST VNCVLAFQGY YLPNDDGEFY QFCYVTHKGE IRGASTPFQF RAASPVEELL TMEDEGNSDM LVVTTKAGLL ELKIEKTLKE KEELLKLIAV LEKETAQLRE QVGRMERELS QEKGRCEQLQ AEQKGLLEVS QSLRVENEEF MKRYSDATAK VQQLEEDIVS VTHKAIEKET DLDSLKDKLR KAQHEREQLE CQLQTEKDEK ELYKVHLKNT EIENTKLVSE IQTLKNLDGN KESMITHFKE EISKLQSCLA DKENLYRALL LTTSNKEDTL FLKEQLRKAE EQVQATRQEL IFLTKELSDA VNVRDKTMAD LHTARLENER VKKQLADTLA ELQLHAVKKD QEKTDTLEHE LRREVEDLKL RLQMAADHYR EKFKECQRLQ KQINKLSDQA ASTNSVFTKK MGSQQKVNDA SINTDPAAST SASAVDVKPA ASCAETGFDM STKDHVCEMT KEIAEKIEKY NKCKQLLQDE KTKCNKYAEE LAKMELKWKE QVKIAENVKL ELAEVEDNYK VQLAEKEKEI NGLASYLENL SREKELTKSL EDQKGRKLEG QSPQQVSRCL NTCSEQNGLL PPLSSAQPVL QYGNPYSAQE TRDGADGAFY PDEIQRPPVR VPSWEDNVVC SQPARNLSRP DGLEDPEDSR EDENVPIPPD PANQHLRSHG AGFCFDSSFD VHKKCPLCEL MFPPNYDQTK FEEHVESHWK VCPMCSEQFP PDYDQQGFER HVQTHFDQNV LNFD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TAX1BP1 (Tax1 (Human T-Cell Leukemia Virus Type I) Binding Protein 1 (TAX1BP1))

Andere Bezeichnung: Tax1bp1 (TAX1BP1 Produkte)

Synonyme: CALCOCO3 Protein, T6BP Protein, TXBP151 Protein, 1200003J11Rik Protein, 1700069J21Rik Protein, AA930106 Protein, D6Ertd404e Protein, D6Ertd772e Protein, T6bp Protein, tax1bp1 Protein, zgc:73219 Protein, zgc:77129 Protein, wu:fc20c12 Protein, wu:fc56e11 Protein, MDP62 Protein, MGC132101 Protein, TAX1BP1 Protein, si:ch211-204j16.2 Protein, Tax1 binding protein 1 Protein, Tax1 (human T cell leukemia virus type I) binding protein 1 Protein, Tax1 (human T-cell leukemia virus type I) binding protein 1b Protein, Tax1 binding protein 1 S homeolog Protein, Tax1 (human T-cell leukemia virus type I) binding protein 1 Protein, Tax1 (human T-cell leukemia virus type I) binding protein 1a Protein, Tax1 binding protein 1 L homeolog Protein, TAX1BP1 Protein, Tax1bp1 Protein, tax1bp1b Protein, tax1bp1.S Protein, tax1bp1a Protein, tax1bp1.L Protein

Hintergrund: Tax1-binding protein 1 homolog,FUNCTION: Ubiquitin-binding adapter that participates in inflammatory, antiviral and innate immune processes as well as selective autophagy regulation (PubMed:18239685, PubMed:33207181). Plays a key role in the negative regulation of NF-kappa-B and IRF3 signalings by acting as an adapter for the ubiquitin-editing enzyme A20/TNFAIP3 to bind and inactivate its substrates. Disrupts the interactions between the E3 ubiquitin ligase TRAF3 and TBK1/IKBKE to attenuate 'Lys63'-linked polyubiquitination of TBK1 and thereby IFN-beta production (By similarity). Recruits also A20/TNFAIP3 to ubiquitinated signaling proteins TRAF6 and RIPK1, leading to their deubiquitination and disruption of IL-1 and TNF-induced NF-kappa-B signaling pathways (PubMed:18239685). Inhibits virus-induced apoptosis by inducing the 'Lys-48'-linked polyubiquitination and degradation of MAVS via recruitment of the E3 ligase ITCH, thereby attenuating MAVS-mediated apoptosis signaling (By similarity). As a macroautophagy/autophagy receptor, facilitates the xenophagic clearance of pathogenic bacteria such as Salmonella typhimurium and Mycobacterium tuberculosis. Upon NBR1 recruitment to the SQSTM1-ubiquitin condensates, acts as the major recruiter of RB1CC1 to these ubiquitin condensates to promote their autophagic degradation (By similarity). Mediates the autophagic degradation of other substrates including TICAM1 (By similarity). {ECO:0000250|UniProtKB:Q86VP1, ECO:0000269|PubMed:18239685, ECO:0000269|PubMed:33207181}.

Molekulargewicht: 93.6 kDa

UniProt: Q3UKC1

Pathways: TLR Signalweg