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ARHGAP17 Protein (AA 1-846) (His tag)

Crystallography grade ARHGAP17 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Produktnummer ABIN3135056
  • Target Alle ARHGAP17 Proteine anzeigen
    ARHGAP17 (rho GTPase Activating Protein 17 (ARHGAP17))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-846
    Spezies
    • 3
    • 1
    Maus
    Quelle
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ARHGAP17 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequenz
    MKKQFNRMKQ LANQTVGRAE KTEVLSEDLL QIERRLDTVR SMCHHSHKRL IACFQGQHGT DAERRHKKLP LTALAQNMQE ASAQLEESLL GKMLETCGDA ENQLALELSQ HEVFVEKEIM DPLYGIAEVE IPNIQKQRKQ LARLVLDWDS VRARWNQAHK SSGTNFQGLP SKIDTLKEEM DEAGNKVEQC KDQLAADMYN FMAKEGEYGK FFVTLLEAQA DYHRKALAVL EKALPEMRAH QDKWAEKPAF GTPLEEHLKR SGREIALPIE ACVMLLLETG MKEEGLFRIG AGASKLKKLK AALDCSTSHL DEFYSDPHAV AGALKSYLRE LPEPLMTFSL YEEWTQVASV QDQDKKLQYL WTTCQKLPPQ NFVNFRYLIK FLAKLAQTSD VNKMTPSNIA IVLGPNLLWA KQEGTLAEIA AATSVHVVAV IEPIIQHADW FFPGEVEFNV SEAFVPLATP NSNHSSHTGN DSDSGTLERK RPASMAVMEG DLVKKESFGV KLMDFQAHRR GGTLNRKHIA PAFQPPLPPT DGNALAPAGP EPPSQSSRAD SSSGGGPVFS STGILEQGLS PGDSSPPKPK DSVSAAVPAA GRNSNQMTTV PNQAQTGGNS HQLSVSTPHS AAGPSPHTLR RAVKKPAPAP PKPGNLPPGH PGGQSSPGTG TSPKPSARSP SPPQQQQQQQ QQQQQQQQQQ TPGMRRCSSS LPPIQAPSHP PPQPPTQPRL GEQGPEPGPT PPQTPTPPST PPLAKQNPSQ SETTQLHGTL PRPRPVPKPR NRPSVPPPPH PPGTHTVDGG LTSSVPTASR IVTDTNSRVS ESLRSIFPEI HSDLASKEVP GHILLDIDND TESTAL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Arhgap17 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ARHGAP17 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ARHGAP17 (rho GTPase Activating Protein 17 (ARHGAP17))
    Andere Bezeichnung
    Arhgap17 (ARHGAP17 Produkte)
    Synonyme
    MST066 Protein, MST110 Protein, MSTP038 Protein, MSTP066 Protein, MSTP110 Protein, NADRIN Protein, PP367 Protein, PP4534 Protein, RICH1 Protein, RICH1B Protein, WBP15 Protein, 5730403H17Rik Protein, Nadrin Protein, Nadrin2 Protein, Rich1 Protein, Wbp15 Protein, Rho GTPase activating protein 17 Protein, ARHGAP17 Protein, arhgap17 Protein, Arhgap17 Protein
    Hintergrund
    Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1 (By similarity). {ECO:0000250}.
    Molekulargewicht
    93.2 kDa Including tag.
    UniProt
    Q3UIA2
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