PUF60 Protein (AA 1-564) (Strep Tag)

Produktnummer ABIN3135030

Produktdetails

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-564

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses PUF60 Protein ist gelabelt mit Strep Tag.

Applikation: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequenz: MATATIALQV NGQQGGGSEP AAAAAAAAAA VVAAGDKWKP PQGTESIKME NGQSTGTKLG LPPLTPEQQE ALQKAKKYAM EQSIKSVLVK QTIAHQQQQL TNLQMAAVTM GFGDPLSPLQ SMAAQRQRAL AIMCRVYVGS IYYELGEDTI RQAFAPFGPI KSIDMSWDSV TMKHKGFAFV EYEVPEAAQL ALEQMNSVML GGRNIKVGRP SNIGQAQPII DQLAEEARAF NRIYVASVHQ DLSDDDIKSV FEAFGKIKSC TLARDPTTGK HKGYGFIEYE KAQSSQDAVS SMNLFDLGGQ YLRVGKAVTP PMPLLTPATP GGLPPAAAVA AAAATAKITA QEAVAGAAVL GTLATPGLVS PALTLAQPLG ALPQAVMAAQ APGVITGVTP ARPPIPVTIP SVGVVNPILA SPPTLGLLEP KKEKEEEELF PESERPEMLS EQEHMSISGS SARHMVMQKL LRKQESTVMV LRNMVDPKDI DDDLEGEVTE ECGKFGAVNR VIIYQEKQGE EEDAEIIVKI FVEFSMASET HKAIQALNGR WFGGRKVVAE VYDQERFDNS DLSA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))

Andere Bezeichnung: Puf60 (PUF60 Produkte)

Synonyme: FIR Protein, RoBPI Protein, SIAHBP1 Protein, fc21a05 Protein, wu:fc21a05 Protein, wu:fi43a08 Protein, 2410104I19Rik Protein, 2810454F19Rik Protein, Siahbp1 Protein, fe37c05 Protein, repressor Protein, si:ch211-12p8.2 Protein, si:zc12p8.2 Protein, wu:fb33e11 Protein, wu:fe37c05 Protein, zgc:86806 Protein, poly(U) binding splicing factor 60 Protein, poly-U binding splicing factor a Protein, poly-U binding splicing factor 60 Protein, poly-U binding splicing factor b Protein, PUF60 Protein, puf60a Protein, Puf60 Protein, puf60b Protein

Hintergrund: Poly(U)-binding-splicing factor PUF60 (60 kDa poly(U)-binding-splicing factor),FUNCTION: DNA- and RNA-binding protein, involved in several nuclear processes such as pre-mRNA splicing, apoptosis and transcription regulation. In association with FUBP1 regulates MYC transcription at the P2 promoter through the core-TFIIH basal transcription factor. Acts as a transcriptional repressor through the core-TFIIH basal transcription factor. Represses FUBP1-induced transcriptional activation but not basal transcription. Decreases ERCC3 helicase activity. Is also involved in pre-mRNA splicing. Promotes splicing of an intron with weak 3'-splice site and pyrimidine tract in a cooperative manner with U2AF2. Involved in apoptosis induction when overexpressed in HeLa cells. Modulates alternative splicing of several mRNAs. Binds to relaxed DNA of active promoter regions. Binds to the pyrimidine tract and 3'-splice site regions of pre-mRNA, binding is enhanced in presence of U2AF2. Binds to Y5 RNA in association with RO60. Binds to poly(U) RNA (By similarity). {ECO:0000250}.

Molekulargewicht: 60.2 kDa

UniProt: Q3UEB3