CAPRIN2 Protein (AA 1-1031) (Strep Tag)

Produktnummer ABIN3134840

Produktdetails

Target: CAPRIN2 (Caprin Family Member 2 (CAPRIN2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1031

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses CAPRIN2 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MKSAKSQVNQ DQQGENQRAL SPLQSTLSSA ASPSQAYETY IDNGLICLKH KIRNIEKKKL KLEDYKDRLK NGEQLNPDQL EAVEKYEEVL HNLEFAKELQ KTFSALSQDL LKAQKKAQRR EHMLKLETEK KKLRTMLQIQ YVLQNLTQEH VQKDFKGGLN GAMYLPSKEL DYLIKFSKLT CPERNESLSV EDQMEQSSLY FWDLLEGSEK TVVGTTYKHV KDLLSKLLHS GYFESVPVLR NSKEKAEEVL MQSEMKKQLL KSESIKESES LTELVQPEIQ PQEFLNRRYM TEVKFSRKQE NVEQSWEADY ARKPSLLKCW NTLPEPDGQE KKKESLESWK SSLKTQEVSK PVVSLVQGKL RPTLQEEQKQ QVPITPVSQW KPESPKSKVG SPQEEQNVQE TPKPWVVQSQ KEQDPKKLPP GSWAVSVQSE QSGSRSWTTP VCREQASVQP GTPVSWENNA ENQKHSLVPQ SQISLKSWGA ASAGLLPNGQ VLTRKLNVEP KDVPKPLPQP IDSSSALPKD PVLRKEKLQD LMSQIQGTYN FMQESVLDFD KPSSAIPSSQ PPSACPVSTV SAEQNLSNQS DFLQEPSQAS SPVTCSSNAC LVTTDQASSG SETEFTTSET PEMVVSPCKP KPASALASPN PPLSKSFQLP PASGSSEAIS TAPFQAMQTV FNVNAPLPPR KEQEMKEPPY SSGYNQNFTS SSTQTVSQCQ LPAVHIDQTT QPPETGAGYH PDGTVQVSNG SLAFYPAPTS MFPRPAQPFI SSRGTLRGCS HGGRLLMSSY QSPGGYKGFD SYRGLPSVSS GNYSQLQLQA REYSGTAYSQ RDNFQQCYKR SGTSSGLQAN SRAGWSDSSQ VSSPERDSET FNSGDSGLGD SRSMTPVDVP VTSPAAAILP VHIYPLPQQM RVAFSAARTS NLAPGTLDQP IVFDLLLNNL GETFDLQLGR FNCPVNGTYV FIFHMLKLAV NVPLYVNLMK NEEVLVSAYA NDGAPDHETA SNHAVLQLLQ GDQIWLRLHR GAIYGSSWKY STFSGYLLYQ D
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CAPRIN2 (Caprin Family Member 2 (CAPRIN2))

Andere Bezeichnung: Caprin2 (CAPRIN2 Produkte)

Synonyme: C1QDC1 Protein, EEG-1 Protein, EEG1 Protein, RNG140 Protein, AA536741 Protein, AV149826 Protein, BC025636 Protein, C1qdc1 Protein, Eeg1 Protein, mKIAA1873 Protein, rng140 Protein, Rng140 Protein, caprin family member 2 Protein, CAPRIN2 Protein, Caprin2 Protein

Hintergrund: Caprin-2 (C1q domain-containing protein 1) (Cytoplasmic activation/proliferation-associated protein 2) (RNA granule protein 140),FUNCTION: Promotes phosphorylation of the Wnt coreceptor LRP6, leading to increased activity of the canonical Wnt signaling pathway (By similarity). Facilitates constitutive LRP6 phosphorylation by CDK14/CCNY during G2/M stage of the cell cycle, which may potentiate cells for Wnt signaling (By similarity). May regulate the transport and translation of mRNAs, modulating for instance the expression of proteins involved in synaptic plasticity in neurons (PubMed:20516077). Involved in regulation of growth as erythroblasts shift from a highly proliferative state towards their terminal phase of differentiation (By similarity). May be involved in apoptosis (By similarity). {ECO:0000250|UniProtKB:Q6IMN6, ECO:0000269|PubMed:20516077}.

Molekulargewicht: 114.5 kDa

UniProt: Q05A80