SMARCAD1 Protein (AA 1-1021) (Strep Tag)

Produktnummer ABIN3134826

Produktdetails

Target: SMARCAD1 (SWI/SNF-Related, Matrix-Associated Actin-Dependent Regulator of Chromatin, Subfamily A, Containing DEAD/H Box 1 (SMARCAD1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-1021

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses SMARCAD1 Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequenz: MNLFNLDRFR FEKRSKIEEA PEAAPQPSQA RPSSPISLSA EEENAEGEGS RANTPDSDVT EKTEDSSVPE PPDNERKASL SCFQNQRAIQ EYIDLSSDTE DVSPNCSSTV QEKKFSKDTV IIVSEPSEDE ESHDLPSVTR RNDSSELEDL SELEDLKDAK LQTLKELFPQ RSDSDLLKLI ESTSTMDGAI AAALLMFGDA GGGPRKRKLS SSSEEDDVND DQSVKQPRGD RGEESNESAE ASSNWEKQES IVLKLQKEFP NFDKQELREV LKEHEWMYTE ALESLKVFAE DQDVQCASQS EVTNGKEVAR NQNYSKNATK IKMKQKISVK PQNGFNKKRK KNVFNPKKAV EDSEYDSGSD AGSSLDEDYS SCEEVMEDGY KGKILHFLQV SSIAELTLIP KCSQKKAQKI TELRPFNNWE ALFTKMSKIN GLSEDLIWNC KTVIQERDVV IRLMNKCEDI SNKLTKQVTM LTGNGGGWNR EQPSLLNQSL SLKPYQKVGL NWLALVHKHG LNGILADEMG LGKTIQAIAF LAYLFQEGNK GPHLIVVPAS TIDNWLREVN LWCPSLNVLC YYGSQEERKQ IRFNIHNKYE DYNVIVTTYN CAISSSDDRS LFRRLKLNYA IFDEGHMLKN MGSIRYQHLM TINARNRLLL TGTPVQNNLL ELMSLLNFVM PHMFSSSTSE IRRMFSSKTK PADEQSIYEK ERIAHAKQII KPFILRRVKE EVLKLLPPKK DRIELCAMSE KQEQLYSGLF NRLKKSINNL EKNTEMCNVM MQLRKMANHP LLHRQYYTPE KLKEMSQLML KEPTHCEANP DLIFEDMEVM TDFELHVLCK QYQHINSYQL DMDLILDSGK FRALGCILSE LKQKGDRVVL FSQFTMMLDI LEVLLKHHQH RYLRLDGKTQ ISERIHLIDE FNTDMDIFVF LLSTKAGGLG INLTSANVVI LHDIDCNPYN DKQAEDRCHR VGQTKEVLVI KLISQGTIEE SMLKINQQKL KLEQDMTTVD EADEGSMPAD IATLLKTSMG L
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SMARCAD1 (SWI/SNF-Related, Matrix-Associated Actin-Dependent Regulator of Chromatin, Subfamily A, Containing DEAD/H Box 1 (SMARCAD1))

Andere Bezeichnung: Smarcad1 (SMARCAD1 Produkte)

Synonyme: etl1 Protein, DKFZp468C0919 Protein, smarcad1a Protein, wu:fi25c01 Protein, zgc:113183 Protein, ADERM Protein, ETL1 Protein, HEL1 Protein, AV081750 Protein, AW226546 Protein, D6Pas1 Protein, Etl1 Protein, mKIAA1122 Protein, SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 Protein, SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 S homeolog Protein, SWI/SNF-related, matrix-associated actin-dependent regulator of chromatin, subfamily a, containing DEAD/H box 1 a Protein, SMARCAD1 Protein, smarcad1.S Protein, smarcad1 Protein, smarcad1a Protein, Smarcad1 Protein

Hintergrund: SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A containing DEAD/H box 1 (EC 3.6.4.12) (ATP-dependent helicase SMARCAD1) (Enhancer trap locus homolog 1) (Etl-1),FUNCTION: DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs. Required for the restoration of heterochromatin organization after replication. Acts at replication sites to facilitate the maintenance of heterochromatin by directing H3 and H4 histones deacetylation, H3 'Lys-9' trimethylation (H3K9me3) and restoration of silencing (By similarity). {ECO:0000250}.

Molekulargewicht: 116.5 kDa

UniProt: Q04692