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CTNNB1 Protein (AA 2-781) (His tag)

Crystallography grade CTNNB1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Produktnummer ABIN3134785
  • Target Alle CTNNB1 Proteine anzeigen
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-781
    Spezies
    • 13
    • 2
    • 2
    Maus
    Quelle
    • 9
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses CTNNB1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    ATQADLMELD MAMEPDRKAA VSHWQQQSYL DSGIHSGATT TAPSLSGKGN PEEEDVDTSQ VLYEWEQGFS QSFTQEQVAD IDGQYAMTRA QRVRAAMFPE TLDEGMQIPS TQFDAAHPTN VQRLAEPSQM LKHAVVNLIN YQDDAELATR AIPELTKLLN DEDQVVVNKA AVMVHQLSKK EASRHAIMRS PQMVSAIVRT MQNTNDVETA RCTAGTLHNL SHHREGLLAI FKSGGIPALV KMLGSPVDSV LFYAITTLHN LLLHQEGAKM AVRLAGGLQK MVALLNKTNV KFLAITTDCL QILAYGNQES KLIILASGGP QALVNIMRTY TYEKLLWTTS RVLKVLSVCS SNKPAIVEAG GMQALGLHLT DPSQRLVQNC LWTLRNLSDA ATKQEGMEGL LGTLVQLLGS DDINVVTCAA GILSNLTCNN YKNKMMVCQV GGIEALVRTV LRAGDREDIT EPAICALRHL TSRHQEAEMA QNAVRLHYGL PVVVKLLHPP SHWPLIKATV GLIRNLALCP ANHAPLREQG AIPRLVQLLV RAHQDTQRRT SMGGTQQQFV EGVRMEEIVE GCTGALHILA RDVHNRIVIR GLNTIPLFVQ LLYSPIENIQ RVAAGVLCEL AQDKEAAEAI EAEGATAPLT ELLHSRNEGV ATYAAAVLFR MSEDKPQDYK KRLSVELTSS LFRTEPMAWN ETADLGLDIG AQGEALGYRQ DDPSYRSFHS GGYGQDALGM DPMMEHEMGG HHPGADYPVD GLPDLGHAQD LMDGLPPGDS NQLAWFDTDL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ctnnb1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product CTNNB1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
    Andere Bezeichnung
    Ctnnb1 (CTNNB1 Produkte)
    Synonyme
    Jup Protein, LOC100217813 Protein, CTNNB1 Protein, CTNNB Protein, Bfc Protein, Catnb Protein, Mesc Protein, ctnnb Protein, id:ibd2058 Protein, wu:fb73e10 Protein, wu:fi81c06 Protein, wu:fk25h01 Protein, ctnnb1 Protein, CHBCAT Protein, MRD19 Protein, armadillo Protein, beta-catenin Protein, catenin beta 1 Protein, catenin (cadherin associated protein), beta 1 Protein, catenin (cadherin-associated protein), beta 1 Protein, catenin beta 1 S homeolog Protein, catenin beta 1 L homeolog Protein, CTNNB1 Protein, Ctnnb1 Protein, ctnnb1 Protein, ctnnb1.S Protein, ctnnb1.L Protein
    Hintergrund
    Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (By similarity). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (PubMed:21325504). {ECO:0000250|UniProtKB:P35222, ECO:0000269|PubMed:21325504}.
    Molekulargewicht
    86.3 kDa Including tag.
    UniProt
    Q02248
    Pathways
    WNT Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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