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ENOS Protein (AA 2-1202) (His tag)

Crystallography grade NOS3 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3134642
  • Target Alle ENOS (NOS3) Proteine anzeigen
    ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-1202
    Spezies
    • 4
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 14
    • 2
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ENOS Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    GNLKSVGQEP GPPCGLGLGL GLGLCGKQGP ASPAPEPSQA PAPPSPTRPA PDHSPPLTRP PDGPRFPRVK NWEVGSITYD TLSAQAQQDG PCTSRRCLGS LVFPRKLQSR PTQGPSPTEQ LLGQARDFIN QYYNSIKRSG SQAHEQRLQE VEAEVAATGT YQLRESELVF GAKQAWRNAP RCVGRIQWGK LQVFDARDCR TAQEMFTYIC NHIKYATNRG NLRSAITVFP QRCPGRGDFR IWNSQLIRYA GYRQQDGSVR GDPANVEITE LCIQHGWTPG NGRFDVLPLL LQAPDEPPEL FTLPPEMVLE VPLEHPTLEW FAALGLRWYA LPAVSNMLLE IGGLEFPAAP FSGWYMSSEI GMRDLCDPHR YNILEDVAVC MDLDTRTTSS LWKDKAAVEI NVAVLHSYQL AKVTIVDHHA ATASFMKHLE NEQKARGGCP ADWAWIVPPI SGSLTPVFHQ EMVNYFLSPA FRYQPDPWKG SAAKGAGITR KKTFKEVANA VKISASLMGT VMAKRVKATI LYGSETGRAQ SYAQQLGRLF RKAFDPRVLC MDEYDVVSLE HEALVLVVTS TFGNGDPPEN GESFAAALME MSGPYNSSPR PEQHKSYKIR FNSVSCSDPL VSSWRRKRKE SSNTDSAGAL GTLRFCVFGL GSRAYPHFCA FARAVDTRLE ELGGERLLQL GQGDELCGQE EAFRGWAQAA FQAACETFCV GEDAKAAARD IFSPKRSWKR QRYRLSTQAE SLQLLPGLTH VHRRKMFQAT ILSVENLQSS KSTRATILVR LDTGGQEGLQ YQPGDHIGVC PPNRPGLVEA LLSRVEDPPP STEPVAVEQL EKGSPGGPPP GWVRDPRLPP CTLRQALTYF LDITSPPSPR LLRLLSTLAE ESSEQQELEA LSQDPRRYEE WKWFSCPTLL EVLEQFPSVA LPAPLILTQL PLLQPRYYSV SSAPSAHPGE IHLTIAVLAY RTQDGLGPLH YGVCSTWMSQ LKAGDPVPCF IRGAPSFRLP PDPNLPCILV GPGTGIAPFR GFWQDRLHDI EIKGLQPAPM TLVFGCRCSQ LDHLYRDEVL DAQQRGVFGQ VLTAFSRDPG SPKTYVQDLL RTELAAEVHR VLCLEQGHMF VCGDVTMATS VLQTVQRILA TEGGMELDEA GDVIGVLRDQ QRYHEDIFGL TLRTQEVTSR IRTQSFSLQE RQLRGAVPWS FDPPGPEIPG S
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Nos3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product NOS3 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))
    Andere Bezeichnung
    Nos3 (NOS3 Produkte)
    Synonyme
    ECNOS Protein, eNOS Protein, 2310065A03Rik Protein, Nos-3 Protein, ecNOS Protein, EC-NOS Protein, NOSIII Protein, cNOS Protein, NOS Protein, NOS3 Protein, eNos Protein, ENOS Protein, nitric oxide synthase 3 Protein, nitric oxide synthase 3, endothelial cell Protein, NOS3 Protein, Nos3 Protein, nos3 Protein
    Hintergrund
    Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development. {ECO:0000269|PubMed:9843834}.
    Molekulargewicht
    133.7 kDa Including tag.
    UniProt
    P70313
    Pathways
    ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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