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PPP1CA Protein (AA 2-330) (His tag)

Crystallography grade PPP1CA Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
Produktnummer ABIN3134581
  • Target Alle PPP1CA Proteine anzeigen
    PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-330
    Spezies
    • 5
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 4
    • 2
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses PPP1CA Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    Sequenz
    SDSEKLNLDS IIGRLLEVQG SRPGKNVQLT ENEIRGLCLK SREIFLSQPI LLELEAPLKI CGDIHGQYYD LLRLFEYGGF PPESNYLFLG DYVDRGKQSL ETICLLLAYK IRYPENFFLL RGNHECASIN RIYGFYDECK RRYNIKLWKT FTDCFNCLPI AAIVDEKIFC CHGGLSPDLQ SMEQIRRIMR PTDVPDQGLL CDLLWSDPDK DVQGWGENDR GVSFTFGAEV VAKFLHKHDL DLICRAHQVV EDGYEFFAKR QLVTLFSAPN YCGEFDNAGA MMSVDETLMC SFQILKPADK NKGKYGQFSG LNPGGRPITP PRNSAKAKK
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Ppp1ca Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product PPP1CA Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PPP1CA (Protein Phosphatase 1, Catalytic Subunit, alpha Isoform (PPP1CA))
    Andere Bezeichnung
    Ppp1ca (PPP1CA Produkte)
    Synonyme
    PP-1A Protein, PP1A Protein, PP1alpha Protein, PPP1A Protein, Ppp1c Protein, dism2 Protein, ppp1a Protein, wu:fc04c08 Protein, wu:fc09b07 Protein, wu:fc30g11 Protein, wu:fe05h08 Protein, zgc:85729 Protein, Ppp1ca Protein, fb18b03 Protein, fd20h04 Protein, wu:fb18b03 Protein, wu:fd20h04 Protein, wu:fl22a03 Protein, zgc:55744 Protein, zgc:76940 Protein, protein phosphatase 1 catalytic subunit alpha Protein, protein phosphatase 1, catalytic subunit, alpha isoform Protein, protein phosphatase 1, catalytic subunit, alpha isozyme L homeolog Protein, protein phosphatase 1, catalytic subunit, alpha isozyme Protein, protein phosphatase 1, catalytic subunit, alpha isozyme a Protein, protein phosphatase 1, catalytic subunit, alpha isozyme b Protein, PPP1CA Protein, Ppp1ca Protein, ppp1ca.L Protein, ppp1ca Protein, ppp1caa Protein, ppp1cab Protein
    Hintergrund
    Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. {ECO:0000269|PubMed:17609112, ECO:0000269|PubMed:21712997, ECO:0000269|PubMed:21930935, ECO:0000269|PubMed:22215812}.
    Molekulargewicht
    38.4 kDa Including tag.
    UniProt
    P62137
    Pathways
    M Phase, Cellular Glucan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Lipid Metabolism
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