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EXOSC10 Protein (AA 1-887) (His tag)

Crystallography grade EXOSC10 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3134522
  • Target Alle EXOSC10 Proteine anzeigen
    EXOSC10 (Exosome Component 10 (EXOSC10))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 1-887
    Spezies
    Maus
    Quelle
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses EXOSC10 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    MAPPSPREHQ SAPATSATKP DAEMVLPGFP DADSFVKFAL GSVVAVTKAS GGLPQFGDEY DFYRSFPAFQ AFCETQGDRL LQCMSRVMQY HGCRSNIKDR SKVTELEDKF DLLVDTNDVI LERVGMLLDE ASGVNKHQQP VLPAGLQVPK TIVSSWNRKA GEYGKKAKSE TFRLLHAKNI VRPQLRFREK IDNSNTPFLP KIFVKPNARK PLPLALSKER RERPQDRPED LDVPPALADF IHQQRTQQVE QDMFAHPYQY ELDHFTPPQS VLQRPKPQLY RAVGETPCHL VSSLDELVEL NEKLLGCQEF AVDLEHHSYR SFLGLTCLMQ ISTRTEDFIV DTLELRSDMY ILNESLTDPA IVKVFHGADS DIEWLQKDFG LYVVNMFDTH QAARLLNLAR HSLDHLLRLY CGVESNKQYQ LADWRIRPLP EEMLSYARDD THYLLYIYDR MRLELWERGN HQPVQLQVVW QRSRDICLKK FVKPIFTDES YLELYRKQKK HLNSQQLTAF QLLFAWRDKT ARREDESYGY VLPNHMMLKI AEELPKEPQG IIACCNPVPP LVRQQINEMH LLIQQAREMP LLKSENAAGV RKSGPLPSAE RLENDLFGPH DCSHAPPDNY QNTSTDGTLP LQKQPSLFTE GKEETSVDAG CLLATAVITL FSEPNTEEGG KTPLTVAQKK AQNIMQSFEN PFRMFLPSLE HKAHISQAAK FDPSSKIYEI SNRWKLASQV QVQKEPKEAT KKKVAEQTAA REEAKEEAAA GVLEQAIPVR QQAALENATK KRERATSDLR TIEQKQEKKR LKSSKKAKDP DPPGKDFSPY DYSQSDFRAF AGDSKSKPSS QFDPNKLAPS GKKGVGAKKC KQSVGNKSMS FAVGKSDRGF RHNWPKR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Exosc10 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product EXOSC10 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    EXOSC10 (Exosome Component 10 (EXOSC10))
    Andere Bezeichnung
    Exosc10 (EXOSC10 Produkte)
    Synonyme
    Pmscl2 Protein, zgc:55695 Protein, pm-scl Protein, pm/scl-100 Protein, pmscl Protein, pmscl2 Protein, rrp6 Protein, rrp6p Protein, EXOSC10 Protein, 201.t00018 Protein, 21.m02990 Protein, DDBDRAFT_0203581 Protein, DDBDRAFT_0233752 Protein, DDB_0203581 Protein, DDB_0233752 Protein, PM-Scl Protein, PM/Scl-100 Protein, PMSCL Protein, PMSCL2 Protein, RRP6 Protein, Rrp6p Protein, p2 Protein, p3 Protein, p4 Protein, exosome component 10 Protein, 3'-5' exonuclease Protein, exosome component 10 L homeolog Protein, exosc10 Protein, EXOSC10 Protein, EHI_021400 Protein, BBOV_IV002650 Protein, CpipJ_CPIJ003896 Protein, CMU_031760 Protein, Tsp_05247 Protein, exosc10.L Protein, Exosc10 Protein
    Hintergrund
    Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA (By similarity). {ECO:0000250}.
    Molekulargewicht
    101.9 kDa Including tag.
    UniProt
    P56960
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