MCM4 Protein (AA 2-862) (His tag)

Produktnummer ABIN3134265

Produktdetails

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-862

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses MCM4 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: SSPASTPSRR SSRRGRVTPT QSLRSEESRS SPNRRRRGED SSTGELLPMP TSPGADLQSP PAQNALFSSP PQMHSLAIPL DFDVSSPLTY GTPSSRVEGT PRSGVRGTPV RQRPDLGSAR KGLQVDLQSD GAAAEDIVPS EQSLGQKLVI WGTDVNVATC KENFQRFLQC FTDPLAKEEE NVGIDITQPL YMQQLGEINI TGEPFLNVNC EHIKSFSKNL YRQLISYPQE VIPTFDMAVN EIFFDRYPDS ILEHQIQVRP FNALKTKSMR NLNPEDIDQL ITISGMVIRT SQLIPEMQEA FFQCQVCAHT TRVEIDRGRI AEPCSCVHCH TTHSMALIHN RSFFSDKQMI KLQESPEDMP AGQTPHTIVL FAHNDLVDKV QPGDRVNVTG IYRAVPIRVN PRVSNVKSVY KTHIDVIHYR KTDAKRLHGL DEEAEQKLFS EKRVKLLKEL SRKPDIYERL ASALAPSIYE HEDIKKGILL QLFGGTRKDF SHTGRGKFRA EINILLCGDP GTSKSQLLQY VYNLVPRGQY TSGKGSSAVG LTAYVMKDPE TRQLVLQTGA LVLSDNGICC IDEFDKMNES TRSVLHEVME QQTLSIAKAG IICQLNARTS VLAAANPIES QWNPKKTTIE NIQLPHTLLS RFDLIFLMLD PQDEAYDRRL AHHLVSLYYQ SEEQVEEEFL DMAVLKDYIA YAHSTIMPRL SEEASQALIE AYVNMRKIGS SRGMVSAYPR QLESLIRLAE AHAKVRFSNK VEAIDVEEAK RLHREALKQS ATDPRTGIVD ISILTTGMSA TSRKRKEELA EALRKLILSK GKTPALKYQQ LFEDIRGQSD TAITKDMFEE ALRALADDDF LTVTGKTVRL L
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Mcm4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Andere Bezeichnung: Mcm4 (MCM4 Produkte)

Synonyme: cdc21 Protein, CDC21 Protein, CDC54 Protein, NKCD Protein, NKGCD Protein, P1-CDC21 Protein, hCdc21 Protein, 19G Protein, AI325074 Protein, AU045576 Protein, Cdc21 Protein, Mcmd4 Protein, mKIAA4003 Protein, mcdc21 Protein, cb1025 Protein, fc12c09 Protein, fj85g09 Protein, hm:zeh1616 Protein, wu:fc12c09 Protein, wu:fj85g09 Protein, zeh1616 Protein, minichromosome maintenance complex component 4 L homeolog Protein, minichromosome maintenance complex component 4 S homeolog Protein, minichromosome maintenance complex component 4 Protein, mcm4.L Protein, mcm4.S Protein, MCM4 Protein, mcm4 Protein, Mcm4 Protein

Hintergrund: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. {ECO:0000250|UniProtKB:P33991}.

Molekulargewicht: 97.6 kDa Including tag.

UniProt: P49717

Pathways: DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA