RANGAP1 Protein (AA 2-589) (His tag)

Produktnummer ABIN3134094

Produktdetails

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-589

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses RANGAP1 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequenz: ASEDIAKLAE TLAKTQVAGG QLSFKGKGLK LNTAEDAKDV IKEIEEFDGL EALRLEGNTV GVEAARVIAK ALEKKSELKR CHWSDMFTGR LRSEIPPALI SLGEGLITAG AQLVELDLSD NAFGPDGVRG FEALLKSPAC FTLQELKLNN CGMGIGGGKI LAAALTECHR KSSAQGKPLA LKVFVAGRNR LENDGATALA EAFGIIGTLE EVHMPQNGIN HPGVTALAQA FAINPLLRVI NLNDNTFTEK GGVAMAETLK TLRQVEVINF GDCLVRSKGA VAIADAVRGG LPKLKELNLS FCEIKRDAAL VVAEAVADKA ELEKLDLNGN ALGEEGCEQL QEVMDSFNMA KVLASLSDDE GEDEDEEEEG EEDDEEEEDE EDEEDDDEEE EEQEEEEEPP QRGSGEEPAT PSRKILDPNS GEPAPVLSSP TPTDLSTFLS FPSPEKLLRL GPKVSVLIVQ QTDTSDPEKV VSAFLKVASV FRDDASVKTA VLDAIDALMK KAFSCSSFNS NTFLTRLLIH MGLLKSEDKI KAIPSLHGPL MVLNHVVRQD YFPKALAPLL LAFVTKPNGA LETCSFARHN LLQTLYNI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Rangap1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RANGAP1 (Ran GTPase Activating Protein 1 (RANGAP1))

Andere Bezeichnung: Rangap1 (RANGAP1 Produkte)

Synonyme: Fug1 Protein, RANGAP Protein, SD Protein, ATRANGAP1 Protein, RAN GTPASE-ACTIVATING PROTEIN 1 Protein, RAN GTPase activating protein 1 Protein, RanGAP1 Protein, fug1 Protein, rangap1b Protein, rna1p Protein, C79654 Protein, mKIAA1835 Protein, rangap1 Protein, rangap1a Protein, Protein rna1 Protein, CG9999 Protein, CT28175 Protein, Dmel\CG9999 Protein, RGP1_DROME Protein, Ran Protein, RanGAP Protein, RanGap Protein, RanGap1 Protein, Scl Protein, Sd Protein, Sd-RanGAP Protein, Sd-RanGap Protein, dRanGAP Protein, ranGAP Protein, ranGap Protein, rangap Protein, zgc:154097 Protein, Ran GTPase activating protein 1 Protein, RAN GTPase activating protein 1 Protein, ran GTPase activating protein 1 Protein, Ran GTPase activating protein 1 S homeolog Protein, Ran GTPase activating protein 1 L homeolog Protein, Ran GAP Rna1 Protein, Ran GTPase activating protein Protein, RAN GTPase activating protein 1a Protein, RANGAP1 Protein, TERG_02084 Protein, rangap1.S Protein, Rangap1 Protein, rangap1.L Protein, rna1 Protein, RanGAP Protein, rangap1a Protein

Hintergrund: GTPase activator for the nuclear Ras-related regulatory protein Ran, converting it to the putatively inactive GDP-bound state. Required for postimplantation development. {ECO:0000269|PubMed:16469311, ECO:0000269|PubMed:9442102, ECO:0000269|PubMed:9456312}.

Molekulargewicht: 64.4 kDa Including tag.

UniProt: P46061

Pathways: M Phase, Protein targeting to Nucleus