TRAF2 Protein (AA 2-501) (His tag)

Produktnummer ABIN3133951

Produktdetails

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-501

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses TRAF2 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: AAASVTSPGS LELLQPGFSK TLLGTRLEAK YLCSACKNIL RRPFQAQCGH RYCSFCLTSI LSSGPQNCAA CVYEGLYEEG ISILESSSAF PDNAARREVE SLPAVCPNDG CTWKGTLKEY ESCHEGLCPF LLTECPACKG LVRLSEKEHH TEQECPKRSL SCQHCRAPCS HVDLEVHYEV CPKFPLTCDG CGKKKIPRET FQDHVRACSK CRVLCRFHTV GCSEMVETEN LQDHELQRLR EHLALLLSSF LEAQASPGTL NQVGPELLQR CQILEQKIAT FENIVCVLNR EVERVAVTAE ACSRQHRLDQ DKIEALSNKV QQLERSIGLK DLAMADLEQK VSELEVSTYD GVFIWKISDF TRKRQEAVAG RTPAIFSPAF YTSRYGYKMC LRVYLNGDGT GRGTHLSLFF VVMKGPNDAL LQWPFNQKVT LMLLDHNNRE HVIDAFRPDV TSSSFQRPVS DMNIASGCPL FCPVSKMEAK NSYVRDDAIF IKAIVDLTGL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Traf2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TRAF2 (TNF Receptor-Associated Factor 2 (TRAF2))

Andere Bezeichnung: Traf2 (TRAF2 Produkte)

Synonyme: MGC:45012 Protein, TRAP Protein, TRAP3 Protein, TANK Protein, AI325259 Protein, TNF-receptor-associated factor 2 Protein, conjugal transfer protein TraF Protein, TNF receptor associated factor 2 Protein, TNF receptor-associated factor 2 Protein, Tnf receptor-associated factor 2 Protein, LOC661716 Protein, traF2 Protein, TRAF2 Protein, Traf2 Protein

Hintergrund: Regulates activation of NF-kappa-B and JNK and plays a central role in the regulation of cell survival and apoptosis. Required for normal antibody isotype switching from IgM to IgG. Has E3 ubiquitin-protein ligase activity and promotes 'Lys-63'-linked ubiquitination of target proteins, such as BIRC3, RIPK1 and TICAM1. Is an essential constituent of several E3 ubiquitin-protein ligase complexes, where it promotes the ubiquitination of target proteins by bringing them into contact with other E3 ubiquitin ligases. Regulates BIRC2 and BIRC3 protein levels by inhibiting their autoubiquitination and subsequent degradation, this does not depend on the TRAF2 RING-type zinc finger domain. Isoform 2 does not seem to mediate activation of NF-kappa-B but inhibits isoform 1 activity. Plays a role in mediating activation of NF-kappa-B by EIF2AK2/PKR. In complex with BIRC2 or BIRC3, promotes ubiquitination of IKBKE. {ECO:0000269|PubMed:10514016, ECO:0000269|PubMed:15121867, ECO:0000269|PubMed:15175328, ECO:0000269|PubMed:18997792, ECO:0000269|PubMed:18997794, ECO:0000269|PubMed:19409903, ECO:0000269|PubMed:19815541, ECO:0000269|PubMed:9390694}.

Molekulargewicht: 56.8 kDa Including tag.

UniProt: P39429

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Unfolded Protein Response, S100 Proteine