Ret Proto-Oncogene (RET) (AA 709-1018) protein (rho-1D4 tag)

Details zu Produkt Nr. ABIN3133865, Anbieter: Anmelden zum Anzeigen
Proteinname
  • c-ret
  • cret
  • etID315074.13
  • ret1
  • wu:fd13h01
  • X-ret
  • ret-A
  • xret
  • RET
  • MTC1
  • CDHF12
  • CDHR16
  • HSCR1
  • MEN2A
  • MEN2B
  • PTC
  • RET-ELE1
  • RET51
  • RET9
  • c-Ret
  • ret proto-oncogene receptor tyrosine kinase
  • ret proto-oncogene
  • ret
  • RET
  • Ret
Proteineigenschaft
AA 709-1018
20
7
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Maus
33
4
Quelle
Insektenzellen
16
15
2
2
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses Ret Proto-Oncogene Protein ist gelabelt mit rho-1D4 tag.
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz SFKIPEDPKW EFPRKNLVLG KTLGEGEFGK VVKATAFRLK GRAGYTTVAV KMLKENASQS ELRDLLSEFN LLKQVNHPHV IKLYGACSQD GPLLLIVEYA KYGSLRGFLR DSRKIGPAYV SGGGSRNSSS LDHPDERVLT MGDLISFAWQ ISRGMQYLAE MKLVHRDLAA RNILVAEGRK MKISDFGLSR DVYEEDSYVK KSKGRIPVKW MAIESLFDHI YTTQSDVWSF GVLLWEIVTL GGNPYPGIPP ERLFNLLKTG HRMERPDNCS EEMYRLMLQC WKQEPDKRPV FADISKDLEK MMVKSRDYLD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Ret Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Reinigung Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin-free.
Güteklasse Crystallography grade
Hintergrund Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis, involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor, in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers, these diseases are characterized by aberrant integrins-regulated cell migration. {ECO:0000269|PubMed:17322904, ECO:0000269|PubMed:20237269}.
Molekulargewicht 36.6 kDa Including tag.
UniProt P35546
Forschungsgebiet Kinases/Phosphatases, Signaling, Tyrosine Kinases, Cancer
Pathways RTK Signalweg, Dopaminergic Neurogenesis, Regulation of Cell Size, Tube Formation
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Ret Proto-Oncogene (RET) (AA 709-1018) protein (rho-1D4 tag) (ABIN3133865) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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