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NOTCH4 Protein (AA 21-1443) (His tag)

Crystallography grade NOTCH4 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
Produktnummer ABIN3133716
  • Target Alle NOTCH4 Proteine anzeigen
    NOTCH4 (Notch 4 (NOTCH4))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 21-1443
    Spezies
    • 5
    • 5
    • 1
    Maus
    Quelle
    • 8
    • 2
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses NOTCH4 Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Sequenz
    RELLCGGSPE PCANGGTCLR LSQGQGICQC APGFLGETCQ FPDPCRDTQL CKNGGSCQAL LPTPPSSRSP TSPLTPHFSC TCPSGFTGDR CQTHLEELCP PSFCSNGGHC YVQASGRPQC SCEPGWTGEQ CQLRDFCSAN PCANGGVCLA TYPQIQCRCP PGFEGHTCER DINECFLEPG PCPQGTSCHN TLGSYQCLCP VGQEGPQCKL RKGACPPGSC LNGGTCQLVP EGHSTFHLCL CPPGFTGLDC EMNPDDCVRH QCQNGATCLD GLDTYTCLCP KTWKGWDCSE DIDECEARGP PRCRNGGTCQ NTAGSFHCVC VSGWGGAGCE ENLDDCAAAT CAPGSTCIDR VGSFSCLCPP GRTGLLCHLE DMCLSQPCHV NAQCSTNPLT GSTLCICQPG YSGSTCHQDL DECQMAQQGP SPCEHGGSCI NTPGSFNCLC LPGYTGSRCE ADHNECLSQP CHPGSTCLDL LATFHCLCPP GLEGRLCEVE VNECTSNPCL NQAACHDLLN GFQCLCLPGF TGARCEKDMD ECSSTPCANG GRCRDQPGAF YCECLPGFEG PHCEKEVDEC LSDPCPVGAS CLDLPGAFFC LCRPGFTGQL CEVPLCTPNM CQPGQQCQGQ EHRAPCLCPD GSPGCVPAED NCPCHHGHCQ RSLCVCDEGW TGPECETELG GCISTPCAHG GTCHPQPSGY NCTCPAGYMG LTCSEEVTAC HSGPCLNGGS CSIRPEGYSC TCLPSHTGRH CQTAVDHCVS ASCLNGGTCV NKPGTFFCLC ATGFQGLHCE EKTNPSCADS PCRNKATCQD TPRGARCLCS PGYTGSSCQT LIDLCARKPC PHTARCLQSG PSFQCLCLQG WTGALCDFPL SCQMAAMSQG IEISGLCQNG GLCIDTGSSY FCRCPPGFQG KLCQDNMNPC EPNPCHHGST CVPQPSGYVC QCAPGYEGQN CSKVLEACQS QPCHNHGTCT SRPGGFHCAC PPGFVGLRCE GDVDECLDRP CHPSGTAACH SLANAFYCQC LPGHTGQRCE VEMDLCQSQP CSNGGSCEIT TGPPPGFTCH CPKGFEGPTC SHKALSCGIH HCHNGGLCLP SPKPGSPPLC ACLSGFGGPD CLTPPAPPGC GPPSPCLHNG TCTETPGLGN PGFQCTCPPD SPGPRCQRPG ASGCEGRGGD GTCDAGCSGP GGDWDGGDCS LGVPDPWKGC PPHSQCWLLF RDGRCHPQCD SEECLFDGYD CEIPLTCIPA YDQYCRDHFH NGHCEKGCNN AECGWDGGDC RPEGEDSEGR PSLALLVVLR PPALDQQLLA LARVLSLTLR VGLWVRKDSE GRNMVFPYPG TRAKEELSGA RDSSSWERQA PPTQPLGKET ESLGAGFVVV MGVDLSRCGP EHPASRCPWD SGLLLRFLAA MAAVGALEPL LPGPLLAAHP QAGTRPPANQ LPW
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Notch4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product NOTCH4 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    NOTCH4 (Notch 4 (NOTCH4))
    Andere Bezeichnung
    Notch4 (NOTCH4 Produkte)
    Synonyme
    INT3 Protein, NOTCH3 Protein, Int-3 Protein, Int3 Protein, N4 Protein, Notch4 Protein, notch 4 Protein, neurogenic locus notch homolog protein 4 Protein, NOTCH4 Protein, Notch4 Protein, LOC100712850 Protein
    Hintergrund
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system. {ECO:0000250, ECO:0000269|PubMed:11344305}.
    Molekulargewicht
    150.4 kDa Including tag.
    UniProt
    P31695
    Pathways
    Notch Signalweg
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