TBP Protein (AA 1-316) (Strep Tag)

Produktnummer ABIN3133614

Produktdetails

Target: TBP (TATA Box Binding Protein (TBP))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-316

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses TBP Protein ist gelabelt mit Strep Tag.

Applikation: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Sequenz: MDQNNSLPPY AQGLASPQGA MTPGIPIFSP MMPYGTGLTP QPIQNTNSLS ILEEQQRQQQ QQQQQQQQQQ AVATAAASVQ QSTSQQPTQG ASGQTPQLFH SQTLTTAPLP GTTPLYPSPM TPMTPITPAT PASESSGIVP QLQNIVSTVN LGCKLDLKTI ALRARNAEYN PKRFAAVIMR IREPRTTALI FSSGKMVCTG AKSEEQSRLA ARKYARVVQK LGFPAKFLDF KIQNMVGSCD VKFPIRLEGL VLTHQQFSSY EPELFPGLIY RMIKPRIVLL IFVSGKVVLT GAKVRAEIYE AFENIYPILK GFRKTT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: TBP (TATA Box Binding Protein (TBP))

Andere Bezeichnung: Tbp (TBP Produkte)

Synonyme: GTF2D Protein, GTF2D1 Protein, HDL4 Protein, SCA17 Protein, TFIID Protein, Gtf2d Protein, fd20h06 Protein, si:zc101n13.2 Protein, wu:fd20h06 Protein, zgc:66213 Protein, TBP1 Protein, TBP Protein, tbp Protein, CG9874 Protein, Dmel\\CG9874 Protein, RBP Protein, TBP38 Protein, TFIIDtau Protein, TfIID Protein, dTBP Protein, dTFIID Protein, dTbp Protein, dtbp Protein, gtf2d Protein, gtf2d1 Protein, sca17 Protein, tfiid Protein, tfiidtau Protein, xtbp Protein, tbp-a Protein, tbp-b Protein, TATA-box binding protein Protein, TATA box binding protein Protein, TATA binding protein Protein, TATA-box binding protein L homeolog Protein, TATA-box-binding protein Protein, TATA-box binding protein S homeolog Protein, TBP Protein, Tbp Protein, tbp Protein, tbp.L Protein, tbp.S Protein

Hintergrund: TATA-box-binding protein (TATA sequence-binding protein) (TATA-binding factor) (TATA-box factor) (Transcription initiation factor TFIID TBP subunit),FUNCTION: General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (pre-initiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. {ECO:0000250|UniProtKB:P20226}.

Molekulargewicht: 34.7 kDa

UniProt: P29037

Pathways: WNT Signalweg