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MAX Protein (AA 2-160) (His tag)

Crystallography grade MAX Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Produktnummer ABIN3133576
  • Target Alle MAX Proteine anzeigen
    MAX (MYC Associated Factor X (MAX))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-160
    Spezies
    • 13
    • 1
    • 1
    • 1
    • 1
    Maus
    Quelle
    • 6
    • 4
    • 4
    • 1
    • 1
    Escherichia coli (E. coli)
    Aufreinigungstag / Konjugat
    Dieses MAX Protein ist gelabelt mit His tag.
    Applikation
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequenz
    SDNDDIEVES DEEQARFQSA ADKRAHHNAL ERKRRDHIKD SFHSLRDSVP SLQGEKASRA QILDKATEYI QYMRRKNDTH QQDIDDLKRQ NALLEQQVRA LEKARSSAQL QTNYPSSDNS LYTNAKGGTI SAFDGGSDSS SESEPEEPQS RKKLRMEAS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Max Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product MAX Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    MAX (MYC Associated Factor X (MAX))
    Andere Bezeichnung
    Max (MAX Produkte)
    Synonyme
    CG9648 Protein, Dmel\\CG9648 Protein, MAX Protein, bHLHd9 Protein, dMax Protein, dmax Protein, max Protein, bhlhd4 Protein, bhlhd5 Protein, bhlhd6 Protein, bhlhd7 Protein, bhlhd8 Protein, xmax2 Protein, xmax4 Protein, xmax Protein, AA960152 Protein, AI875693 Protein, bHLHd4 Protein, bHLHd5 Protein, bHLHd6 Protein, bHLHd7 Protein, bHLHd8 Protein, fa92b04 Protein, fb52a04 Protein, fb95h11 Protein, wu:fa92b04 Protein, wu:fb52a04 Protein, wu:fb95h11 Protein, zmax Protein, CG9648 gene product from transcript CG9648-RD Protein, MYC associated factor X L homeolog Protein, MYC associated factor X Protein, Max protein Protein, MYC associated factor X S homeolog Protein, myc associated factor X Protein, Max Protein, max.L Protein, MAX Protein, max Protein, max.S Protein
    Hintergrund
    Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. CpG methylation of the recognition site greatly inhibits DNA binding, suggesting that DNA methylation may regulate the MYC:MAX complex in vivo. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity.
    Molekulargewicht
    19.0 kDa Including tag.
    UniProt
    P28574
    Pathways
    Mitotic G1-G1/S Phases
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