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PIK3R1 Protein (AA 2-724) (His tag)

Crystallography grade PI3K p85a Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, Crys, SDS
Produktnummer ABIN3133475
  • Target Alle PIK3R1 (PI3K p85a) Proteine anzeigen
    PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 2-724
    Spezies
    Maus
    Quelle
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses PIK3R1 Protein ist gelabelt mit His tag.
    Applikation
    Western Blotting (WB), ELISA, Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    SAEGYQYRAL YDYKKEREED IDLHLGDILT VNKGSLVALG FSDGQEARPE DIGWLNGYNE TTGERGDFPG TYVEYIGRKR ISPPTPKPRP PRPLPVAPGS SKTEADTEQQ ALPLPDLAEQ FAPPDVAPPL LIKLLEAIEK KGLECSTLYR TQSSSNPAEL RQLLDCDAAS VDLEMIDVHV LADAFKRYLA DLPNPVIPVA VYNEMMSLAQ ELQSPEDCIQ LLKKLIRLPN IPHQCWLTLQ YLLKHFFKLS QASSKNLLNA RVLSEIFSPV LFRFPAASSD NTEHLIKAIE ILISTEWNER QPAPALPPKP PKPTTVANNS MNNNMSLQDA EWYWGDISRE EVNEKLRDTA DGTFLVRDAS TKMHGDYTLT LRKGGNNKLI KIFHRDGKYG FSDPLTFNSV VELINHYRNE SLAQYNPKLD VKLLYPVSKY QQDQVVKEDN IEAVGKKLHE YNTQFQEKSR EYDRLYEEYT RTSQEIQMKR TAIEAFNETI KIFEEQCQTQ ERYSKEYIEK FKREGNEKEI QRIMHNHDKL KSRISEIIDS RRRLEEDLKK QAAEYREIDK RMNSIKPDLI QLRKTRDQYL MWLTQKGVRQ KKLNEWLGNE NTEDQYSLVE DDEDLPHHDE KTWNVGSSNR NKAENLLRGK RDGTFLVRES SKQGCYACSV VVDGEVKHCV INKTATGYGF AEPYNLYSSL KELVLHYQHT SLVQHNDSLN VTLAYPVYAQ QRR
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Pik3r1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product PI3K p85a Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    PIK3R1 (PI3K p85a) (Phosphoinositide 3 Kinase, p85 alpha (PI3K p85a))
    Andere Bezeichnung
    Pik3r1 (PI3K p85a Produkte)
    Synonyme
    PI3CG Protein, PI3K Protein, PI3Kgamma Protein, PIK3 Protein, AGM7 Protein, GRB1 Protein, p85 Protein, p85-ALPHA Protein, PI3KA Protein, AA414921 Protein, C530050K14 Protein, p50alpha Protein, p55alpha Protein, p85alpha Protein, wu:fb65b05 Protein, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma Protein, phosphoinositide-3-kinase regulatory subunit 1 Protein, phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta Protein, phosphoinositide-3-kinase, regulatory subunit 1 (alpha) Protein, PIK3CG Protein, PIK3R1 Protein, Pik3r1 Protein, Pik3cb Protein, pik3r1 Protein
    Hintergrund
    Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926). {ECO:0000250, ECO:0000269|PubMed:20348926}.
    Molekulargewicht
    84.3 kDa Including tag.
    UniProt
    P26450
    Pathways
    T-Zell Rezeptor Signalweg, Response to Growth Hormone Stimulus, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Hepatitis C, Protein targeting to Nucleus, VEGF Signaling, BCR Signaling, Warburg Effekt
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