RPL13A Protein (AA 2-203) (His tag)

Produktnummer ABIN3133220

Produktdetails

Target: RPL13A (Ribosomal Protein L13a (RPL13A))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-203

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses RPL13A Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: AEGQVLVLDG RGHLLGRLAA IVAKQVLLGR KVVVVRCEGI NISGNFYRNK LKYLAFLRKR MNTNPSRGPY HFRAPSRIFW RTVRGMLPHK TKRGQAALER LKVLDGIPPP YDKKKRMVVP AALKVVRLKP TRKFAYLGRL AHEVGWKYQA VTATLEEKRK EKAKMHYRKK KQILRLRKQA EKNVEKKICK FTEVLKTNGL LV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Rpl13a Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: RPL13A (Ribosomal Protein L13a (RPL13A))

Andere Bezeichnung: Rpl13a (RPL13A Produkte)

Synonyme: 1810026N22Rik Protein, Tstap198-7 Protein, tum-antigen Protein, L13A Protein, TSTA1 Protein, CG1475 Protein, Dmel\\CG1475 Protein, L13a2 Protein, M(3)83B Protein, Rp L13A Protein, anon-EST:Posey125 Protein, anon-EST:fe1A5 Protein, bs25f04.y1 Protein, cg1475 Protein, MGC54018 Protein, GB16111 Protein, hm:zehp0384 Protein, wu:fa95e06 Protein, wu:fb08g07 Protein, wu:fd05d08 Protein, DDBDRAFT_0217704 Protein, DDBDRAFT_0231192 Protein, DDB_0217704 Protein, DDB_0231192 Protein, ribosomal protein L13A Protein, ribosomal protein L13a Protein, Ribosomal protein L13A Protein, ribosomal protein L13a S homeolog Protein, 60S ribosomal protein L13a Protein, ribosomal protein 13a Protein, ribosomal 60S subunit protein L13A Protein, Ribosomal protein L13a, component of cytosolic 80S ribosome and 60S large subunit Protein, S60 ribosomal protein L13a Protein, Rpl13a Protein, RPL13A Protein, RpL13A Protein, rpl13a.S Protein, rpl13a Protein, LOC551418 Protein, LOC663151 Protein, RPL13a Protein

Hintergrund: Associated with ribosomes but is not required for canonical ribosome function and has extra-ribosomal functions (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma activation and subsequent phosphorylation dissociates from the ribosome and assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. In the GAIT complex interacts with m7G cap-bound eIF4G at or near the eIF3-binding site and blocks the recruitment of the 43S ribosomal complex. {ECO:0000250, ECO:0000269|PubMed:23071094}.

Molekulargewicht: 24.3 kDa Including tag.

UniProt: P19253