KRT8 Protein (AA 1-490) (Strep Tag)

Produktnummer ABIN3132867

Produktdetails

Target: KRT8 (Keratin 8 (KRT8))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-490

Spezies: Maus

Quelle: Tobacco (Nicotiana tabacum)

Aufreinigungstag / Konjugat: Dieses KRT8 Protein ist gelabelt mit Strep Tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Sequenz: MSIRVTQKSY KMSTSGPRAF SSRSFTSGPG ARISSSSFSR VGSSSSSFRG SMGTGVGLGG FGGAGVGGIT AVTVNQSLLS PLKLEVDPNI QAVRTQEKEQ IKSLNNKFAS FIDKVRFLEQ QNKMLETKWS LLQQQKTSRS NMDNMFESYI NNLRRQLEAL GQEKLKLEAE LGNMQGLVED FKNKYEDEIN KRTEMENEFV LIKKDVDEAY MNKVELESRL EGLTDEINFL RQIHEEEIRE LQSQISDTSV VLSMDNSRSL DMDGIIAEVR AQYEDIANRS RAEAETMYQI KYEELQTLAG KHGDDLRRTK TEISEMNRNI NRLQAEIEAL KGQRASLEAA IADAEQRGEM AIKDAQTKLA ELEAALQRAK QDMARQLREY QELMNVKLAL DIEITTYRKL LEGEESRLES GMQNMSIHTK TTSGYSGGLS SSYGGLTSPG FSYGMSSFQP GFGSAGGSNT FSRTTKAVVV KKIETRDGKL VSESSDVVSK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Produktmerkmale: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin-Niveau: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Kommentare:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: KRT8 (Keratin 8 (KRT8))

Andere Bezeichnung: Krt8 (KRT8 Produkte)

Synonyme: CARD2 Protein, CK-8 Protein, CK8 Protein, CYK8 Protein, K2C8 Protein, K8 Protein, KO Protein, AA960620 Protein, AL022697 Protein, AU019895 Protein, Card2 Protein, EndoA Protein, Krt-2.8 Protein, Krt2-8 Protein, CYKER Protein, KRT2-8 Protein, KERATIN8 Protein, ck8 Protein, cyk8 Protein, k2c8 Protein, card2 Protein, krt2-5 Protein, MGC69490 Protein, KRT8 Protein, DreK8 Protein, cb186 Protein, krt2-8 Protein, sb:cb186 Protein, wu:fa20h05 Protein, wu:fa95h10 Protein, wu:fb96c06 Protein, zf-K8 Protein, zfk8 Protein, DKFZp468F2127 Protein, keratin 8 Protein, keratin 8 S homeolog Protein, KRT8 Protein, Krt8 Protein, krt8.S Protein, krt8 Protein

Hintergrund: Keratin, type II cytoskeletal 8 (Cytokeratin endo A) (Cytokeratin-8) (CK-8) (Keratin-8) (K8) (Type-II keratin Kb8),FUNCTION: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. {ECO:0000250}.

Molekulargewicht: 54.6 kDa

UniProt: P11679