Surfeit 1 (SURF1) (AA 1-306) protein (rho-1D4 tag)

Details zu Produkt Nr. ABIN3132711, Anbieter: Anmelden zum Anzeigen
Proteinname
  • BcDNA:GH23574
  • CG9943
  • Dmel\\CG9943
  • GB14201
  • SURF1
  • im:6898613
  • zgc:158646
  • Ab1-205
  • SURF-1
  • 0610010F23Rik
  • Surf-1
  • SURFEIT 1
  • Surfeit 1
  • surfeit 1
  • surfeit gene 1
  • Surfeit locus 1 cytochrome c oxidase biogenesis protein
  • Surf1
  • SURF1
  • surf1
Proteineigenschaft
AA 1-306
2
2
1
1
Spezies
Maus
8
1
Quelle
Insektenzellen
4
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses SURF1 Protein ist gelabelt mit rho-1D4 tag.
Applikation
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Sequenz MAAVMALAVL PRRMTRWSQW AYAGRAQFCA VRRSVFGFSV RSGMVCRPRR CCSSTAETAA AKAEDDSFLQ WFLLLIPATA FGLGTWQVQR RKWKLKLIAE LESRVMAEPI PLPADPMELK NLEYRPVKVR GHFDHSKELY IMPRTMVDPV REARDAGRLS STESGAHVVT PFHCSDLGVT ILVNRGFVPR KKVNPETRQK GQVLGEVDLV GIVRLTENRK PFVPENSPER NHWYYRDLEA MAKITGADPI FIDADFHSTA PGGPIGGQTR VTLRNEHMQY ILTWYGLCAA TSYLWFQKFV RRTPIM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Produktmerkmale
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Surf1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).
Reinigung Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
  1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
  2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
  3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Reinheit >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterilität 0.22 μm filtered
Endotoxin-Niveau Protein is endotoxin-free.
Güteklasse Crystallography grade
Hintergrund Probably involved in the biogenesis of the COX complex. {ECO:0000250}.
Molekulargewicht 36.0 kDa Including tag.
UniProt P09925
Forschungsgebiet Signaling, Organelles, Metabolism
Pathways Proton Transport, Ribonucleoside Biosynthetic Process
Applikationshinweise In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Kommentare

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -80 °C
Informationen zur Lagerung Store at -80°C.
Haltbarkeit Unlimited (if stored properly)
Bilder des Herstellers
 image for Surfeit 1 (SURF1) (AA 1-306) protein (rho-1D4 tag) (ABIN3132711) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
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