Nucleolin Protein (NCL) (AA 2-707) (His tag)

Produktnummer ABIN3132676

Produktdetails

Target: Nucleolin (NCL)

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-707

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses Nucleolin Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: VKLAKAGKTH GEAKKMAPPP KEVEEDSEDE EMSEDEDDSS GEEEVVIPQK KGKKATTTPA KKVVVSQTKK AAVPTPAKKA AVTPGKKAVA TPAKKNITPA KVIPTPGKKG AAQAKALVPT PGKKGAATPA KGAKNGKNAK KEDSDEDEDE EDEDDSDEDE DDEEEDEFEP PIVKGVKPAK AAPAAPASED EEDDEDEDDE EDDDEEEEDD SEEEVMEITT AKGKKTPAKV VPMKAKSVAE EEDDEEEDED DEDEDDEEED DEDDDEEEEE EEPVKAAPGK RKKEMTKQKE APEAKKQKVE GSEPTTPFNL FIGNLNPNKS VNELKFAISE LFAKNDLAVV DVRTGTNRKF GYVDFESAED LEKALELTGL KVFGNEIKLE KPKGRDSKKV RAARTLLAKN LSFNITEDEL KEVFEDAMEI RLVSQDGKSK GIAYIEFKSE ADAEKNLEEK QGAEIDGRSV SLYYTGEKGQ RQERTGKTST WSGESKTLVL SNLSYSATKE TLEEVFEKAT FIKVPQNPHG KPKGYAFIEF ASFEDAKEAL NSCNKMEIEG RTIRLELQGS NSRSQPSKTL FVKGLSEDTT EETLKESFEG SVRARIVTDR ETGSSKGFGF VDFNSEEDAK AAKEAMEDGE IDGNKVTLDW AKPKGEGGFG GRGGGRGGFG GRGGGRGGRG GFGGRGRGGF GGRGGFRGGR GGGGDFKPQG KKTKFE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ncl Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: Nucleolin (NCL)

Andere Bezeichnung: Ncl (NCL Produkte)

Synonyme: C23 Protein, B530004O11Rik Protein, D0Nds28 Protein, D1Nds28 Protein, Nucl Protein, wu:fa12d03 Protein, zgc:152810 Protein, nucl Protein, LOC100282923 Protein, LOC100283058 Protein, nucleolin Protein, nucleolin S homeolog Protein, NCL Protein, Ncl Protein, ncl.S Protein, ncl Protein, LOC100195537 Protein, LOC100282923 Protein, LOC100283058 Protein

Hintergrund: Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats (By similarity). {ECO:0000250, ECO:0000269|PubMed:8065340}.

Molekulargewicht: 77.5 kDa Including tag.

UniProt: P09405

Pathways: Ribonucleoprotein Complex Subunit Organization