CD11b Protein (AA 17-1105) (His tag)

Produktnummer ABIN3132526

Produktdetails

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Protein-Typ: Recombinant

Proteineigenschaft: AA 17-1105

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses CD11b Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: FNLDTEHPMT FQENAKGFGQ NVVQLGGTSV VVAAPQEAKA VNQTGALYQC DYSTSRCHPI PLQVPPEAVN MSLGLSLAVS TVPQQLLACG PTVHQNCKEN TYVNGLCYLF GSNLLRPPQQ FPEALRECPQ QESDIVFLID GSGSINNIDF QKMKEFVSTV MEQFKKSKTL FSLMQYSDEF RIHFTFNDFK RNPSPRSHVS PIKQLNGRTK TASGIRKVVR ELFHKTNGAR ENAAKILVVI TDGEKFGDPL DYKDVIPEAD RAGVIRYVIG VGNAFNKPQS RRELDTIASK PAGEHVFQVD NFEALNTIQN QLQEKIFAIE GTQTGSTSSF EHEMSQEGFS ASITSNGPLL GSVGSFDWAG GAFLYTSKDK VTFINTTRVD SDMNDAYLGY ASAVILRNRV QSLVLGAPRY QHIGLVVMFR ENFGTWEPHT SIKGSQIGSY FGASLCSVDM DADGNTNLIL IGAPHYYEKT RGGQVSVCPL PRGRARWQCE ALLHGDQGHP WGRFGAALTV LGDVNGDKLT DVAIGAPGEQ ENQGAVYIFY GASIASLSAS HSHRIIGAHF SPGLQYFGQS LSGGKDLTMD GLMDLAVGAQ GHLLLLRAQP VLRLEATMEF SPKKVARSVF ACQEQVLKNK DAGEVRVCLR VRKNTKDRLR EGDIQSTVTY DLALDPVRSR IRAFFDETKN NTRRRTQVFG LMQKCETLKL ILPDCVDDSV SPIILRLNYT LVGEPLRSFG NLRPVLAMDA QRFFTAMFPF EKNCGNDSIC QDDLSITMSA MGLDTLVVGG PQDFNMSVTL RNDGEDSYGT QVTVYYPSGL SYRKDSASQN PLTKKPWFVK PAESSSSSEG HGALKSTTWN INHPIFPANS EVTFNVTFDV DSHASFGNKL LLKAIVASEN NMSRTHKTKF QLELPVKYAI YMIVTSDESS IRYLNFTASE MTSKVIQHQY QFNNLGQRSL PVSVVFWIPV QINNVTVWDH PQVIFSQNLS SACHTEQKSP PHSNFRDQLE RTPVLNCSVA VCKRIQCDLP SFNTQEIFNV TLKGNLSFDW YIKTSHGHLL LVSSTEILFN DSAFALLPGQ ESYVRSKTET KVEPYEVHN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Itgam Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: CD11b (ITGAM) (Integrin alpha M (ITGAM))

Andere Bezeichnung: Itgam (ITGAM Produkte)

Synonyme: CD11b/CD18 Protein, CR3 Protein, CR3A Protein, Cd11b Protein, F730045J24Rik Protein, Ly-40 Protein, MAC1 Protein, Mac-1 Protein, Mac-1a Protein, CD11B Protein, MAC-1 Protein, MAC1A Protein, MO1A Protein, SLEB6 Protein, C3bi Protein, integrin subunit alpha M Protein, integrin alpha M Protein, integrin, alpha M (complement component 3 receptor 3 subunit) Protein, integrin alpha-M Protein, ITGAM Protein, Itgam Protein, LOC100351865 Protein

Hintergrund: Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Alpha-M/beta-2 play a critical role in mast cell development and in immune complex-mediated glomerulonephritis. Mice expressing a null mutation of the alpha-M subunit gene demonstrate increase in neutrophil accumulation, in response to a impaired degranulation and phagocytosis, events that apparently accelerate apoptosis in neutrophils. These mice develop obesity.

Molekulargewicht: 121.7 kDa Including tag.

UniProt: P05555

Pathways: Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation