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ROBO1 Protein (AA 880-1612) (His tag)

Crystallography grade ROBO1 Spezies: Maus Wirt: Insektenzellen Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Produktnummer ABIN3132300
  • Target Alle ROBO1 Proteine anzeigen
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Protein-Typ
    Recombinant
    Proteineigenschaft
    AA 880-1612
    Spezies
    • 9
    • 2
    • 1
    Maus
    Quelle
    • 5
    • 5
    • 1
    • 1
    Insektenzellen
    Aufreinigungstag / Konjugat
    Dieses ROBO1 Protein ist gelabelt mit His tag.
    Applikation
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequenz
    YRHRKKRNGL TSTYAGIRKV PSFTFTPTVT YQRGGEAVSS GGRPGLLNIS EPATQPWLAD TWPNTGNNHN DCSINCCTAG NGNSDSNLTT YSRPADCIAN YNNQLDNKQT NLMLPESTVY GDVDLSNKIN EMKTFNSPNL KDGRFVNPSG QPTPYATTQL IQANLSNNMN NGAGDSSEKH WKPPGQQKPE VAPIQYNIME QNKLNKDYRA NDTIPPTIPY NQSYDQNTGG SYNSSDRGSS TSGSQGHKKG ARTPKAPKQG GMNWADLLPP PPAHPPPHSN SEEYNMSVDE SYDQEMPCPV PPAPMYLQQD ELQEEEDERG PTPPVRGAAS SPAAVSYSHQ STATLTPSPQ EELQPMLQDC PEDLGHMPHP PDRRRQPVSP PPPPRPISPP HTYGYISGPL VSDMDTDAPE EEEDEADMEV AKMQTRRLLL RGLEQTPASS VGDLESSVTG SMINGWGSAS EEDNISSGRS SVSSSDGSFF TDADFAQAVA AAAEYAGLKV ARRQMQDAAG RRHFHASQCP RPTSPVSTDS NMSAVVIQKA RPAKKQKHQP GHLRREAYAD DLPPPPVPPP AIKSPTVQSK AQLEVRPVMV PKLASIEART DRSSDRKGGS YKGREALDGR QVTDLRTNPS DPREAQEQPN DGKGRGTRQP KRDLPPAKTH LGQEDILPYC RPTFPTSNNP RDPSSSSSMS SRGSGSRQRE QANVGRRNMA EMQVLGGFER GDENNEELEE TES
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Produktmerkmale
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Robo1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Aufreinigung
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Reinheit
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterilität
    0.22 μm filtered
    Endotoxin-Niveau
    Protein is endotoxin free.
    Güteklasse
    Crystallography grade
    Top Product
    Discover our top product ROBO1 Protein
  • Applikationshinweise
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Kommentare

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handhabung
    Avoid repeated freeze-thaw cycles.
    Lagerung
    -80 °C
    Informationen zur Lagerung
    Store at -80°C.
    Haltbarkeit
    Unlimited (if stored properly)
  • Target
    ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
    Andere Bezeichnung
    Robo1 (ROBO1 Produkte)
    Synonyme
    DUTT1 Protein, SAX3 Protein, AW494633 Protein, AW742721 Protein, Gm310 Protein, ROBO1 Protein, dutt1 Protein, roundabout Protein, sax3 Protein, MGC122264 Protein, roundabout-1 Protein, roundabout guidance receptor 1 Protein, roundabout, axon guidance receptor, homolog 1 (Drosophila) Protein, roundabout guidance receptor 1 L homeolog Protein, ROBO1 Protein, Robo1 Protein, robo1 Protein, robo1.L Protein
    Hintergrund
    Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10433822, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B, inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (By similarity). May be required for lung development (PubMed:11734623). {ECO:0000250|UniProtKB:Q9Y6N7, ECO:0000269|PubMed:10433822, ECO:0000269|PubMed:11734623, ECO:0000269|PubMed:15091338, ECO:0000269|PubMed:24560577}.
    Molekulargewicht
    80.9 kDa Including tag.
    UniProt
    O89026
    Pathways
    Positive Regulation of Endopeptidase Activity
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