ASNA1 Protein (AA 2-348) (His tag)

Produktnummer ABIN3131833

Produktdetails

Target: ASNA1 (ArsA Arsenite Transporter, ATP-Binding, Homolog 1 (ASNA1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-348

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses ASNA1 Protein ist gelabelt mit His tag.

Applikation: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequenz: AAGVAGWGVE AEEFEDAPDV EPLEPTLSNI IEQRSLKWIF VGGKGGVGKT TCSCSLAVQL SKGRESVLII STDPAHNISD AFDQKFSKVP TKVKGYDNLF AMEIDPSLGV AELPDEFFEE DNMLSMGKKM MQEAMSAFPG IDEAMSYAEV MRLVKGMNFS VVVFDTAPTG HTLRLLNFPT IVERGLGRLM QIKNQISPFI SQMCNMLGLG DMNADQLASK LEETLPVIRS VSEQFKDPEQ TTFICVCIAE FLSLYETERL IQELAKCKID THNIIVNQLV FPDPEKPCKM CEARHKIQAK YLDQMEDLYE DFHIVKLPLL PHEVRGADKV NTFSALLLEP YKPPSTQ
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Asna1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: ASNA1 (ArsA Arsenite Transporter, ATP-Binding, Homolog 1 (ASNA1))

Andere Bezeichnung: Asna1 (ASNA1 Produkte)

Synonyme: ARSA-I Protein, ARSA1 Protein, ASNA-I Protein, GET3 Protein, TRC40 Protein, hASNA-I Protein, 1810048H22Rik Protein, ArsA Protein, wu:fd05d03 Protein, zgc:56540 Protein, zgc:86799 Protein, arsa-i Protein, arsa1 Protein, ASNA1 Protein, asna1 Protein, MGC146316 Protein, arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) Protein, arsenical pump-driving ATPase Protein, pseudo Protein, ATPase ASNA1 homolog Protein, arsenite-translocating ATPase; arsenical resistance ATPase; arsenite-transporting ATPase Protein, arsA arsenite transporter, ATP-binding, homolog 1 (bacterial) S homeolog Protein, L-asparaginase Protein, ATPase asna-1 Protein, ASNA1 Protein, ATEG_04670 Protein, arsA2 Protein, arsA Protein, HCAG_00048 Protein, LOC5574421 Protein, CC1G_08655 Protein, CpipJ_CPIJ005690 Protein, PTRG_02265 Protein, pKlebpneu12_p77 Protein, UREG_07455 Protein, BDBG_06308 Protein, MCYG_00630 Protein, LOC100284977 Protein, Asna1 Protein, asna1 Protein, asna1.S Protein, MGYG_01332 Protein, asnA1 Protein, Tsp_06776 Protein, asna-1 Protein

Hintergrund: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. {ECO:0000255|HAMAP-Rule:MF_03112}.

Molekulargewicht: 39.6 kDa Including tag.

UniProt: O54984

Pathways: Positive Regulation of Peptide Hormone Secretion