Bystin-Like Protein (BYSL) (AA 1-436) (His tag)
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- Target Alle Bystin-Like (BYSL) Proteine anzeigen
- Bystin-Like (BYSL)
- Protein-Typ
- Recombinant
- Proteineigenschaft
- AA 1-436
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Spezies
- Maus
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Quelle
- Insektenzellen
- Aufreinigungstag / Konjugat
- Dieses Bystin-Like Protein ist gelabelt mit His tag.
- Applikation
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequenz
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MPKFKVTRGA SNREKHAPLA EQILAGNAVR AGTREKRRGR EVEEEEEYVG PRLSRRILQQ ARQQQEELET DHGAGDRSAP PRERATRLGP GLPQDGSDEE DEEWPTLEKA AKMAGVDHQA EVIVDPEDER AIEMFMNKNP PVRRTLADII MEKLTEKQTE VETVMSEVSG FPMPQLDPRV LEVYRGVREV LCKYRSGKLP KAFKVIPALS NWEQILYVTE PEAWTAAAMY QATRIFASNL KERMAQRFYN LVLLPRVRDD IAEYKRLNFH LYMALKKALF KPGAWFKGIL IPLCESGTCT LREAIIVGSI ITKCSIPVLH SSAAMLKIAE MEYSGANSIF LRLLLDKKYA LPYRVLDALV FHFLAFRTEK RQLPVLWHQC LLTLAQRYKA DLATEQKEAL LELLRLQPHP QLSPEIRREL QSAVPRDVED GGVTME
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Produktmerkmale
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Bysl Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Aufreinigung
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Reinheit
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Protein is endotoxin free.
- Güteklasse
- Crystallography grade
- Top Product
- Discover our top product BYSL Protein
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- Applikationshinweise
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Kommentare
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handhabung
- Avoid repeated freeze-thaw cycles.
- Lagerung
- -80 °C
- Informationen zur Lagerung
- Store at -80°C.
- Haltbarkeit
- Unlimited (if stored properly)
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- Target
- Bystin-Like (BYSL)
- Andere Bezeichnung
- Bysl (BYSL Produkte)
- Synonyme
- BYSTIN Protein, Bys Protein, Enp1 Protein, MGC63889 Protein, zgc:63889 Protein, MGC81422 Protein, MGC97811 Protein, CG1430 Protein, Dmel\\CG1430 Protein, bystin like Protein, bystin-like Protein, putative bystin Protein, bystin Protein, bystin like S homeolog Protein, by S6 Protein, BYSL Protein, Bysl Protein, bysl Protein, LMJF_30_0480 Protein, MCYG_02768 Protein, LOC100270780 Protein, bysl.S Protein, LOC100159994 Protein, bys Protein
- Hintergrund
- Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. {ECO:0000269|PubMed:17055491, ECO:0000269|PubMed:17242206}.
- Molekulargewicht
- 50.7 kDa Including tag.
- UniProt
- O54825
- Pathways
- Cellular Response to Molecule of Bacterial Origin
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