SPTLC1 Protein (AA 37-473) (His tag)

Produktnummer ABIN3131666

Produktdetails

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 37-473

Spezies: Maus

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses SPTLC1 Protein ist gelabelt mit His tag.

Applikation: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequenz: RLVFSKTYKL QERSDLTAKE KEELIEEWQP EPLVPPVSKN HPALNYNIVS GPPTHNIVVN GKECVNFASF NFLGLLANPR VKATAFSSLK KYGVGTCGPR GFYGTFDVHL DLEERLAKFM KTEEAIIYSY GFSTIASAIP AYSKRGDIIF VDSAACFAIQ KGLQASRSDI KLFKHNDVAD LERLLKEQEI EDQKNPRKAR VTRRFIVVEG LYMNTGTICP LPELVKLKYK YKARIFLEES LSFGVLGEHG RGVTEHYGIS IDDIDLISAN MENALASVGG FCCGRSFVVD HQRLSGQGYC FSASLPPLLA AAAIEALNIM EENPDIFAVL KKKCQNIHKS LQGVSGLKVV GESLSPALHL QLEESTGSRE KDVKLLQAIV DQCMDKGIAL TQARYLDKEE KCLPPPSIRV VVTVEQTEEE LQRAASTIRE AAQAVLL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sptlc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: SPTLC1 (serine Palmitoyltransferase, Long Chain Base Subunit 1 (SPTLC1))

Andere Bezeichnung: Sptlc1 (SPTLC1 Produkte)

Synonyme: ATSPT1 Protein, SERINE PALMITOYLTRANSFERASE 1 Protein, serine palmitoyltransferase 1 Protein, Afu6g00300 Protein, HSAN1 Protein, HSN1 Protein, LBC1 Protein, LCB1 Protein, SPT1 Protein, SPTI Protein, wu:fc75h04 Protein, wu:fp41c08 Protein, zgc:112247 Protein, AW552086 Protein, C77762 Protein, E030036H05 Protein, Lcb1 Protein, RGD1306617 Protein, Spt1 Protein, serine palmitoyltransferase 1 Protein, Serine palmitoyltransferase 1 Protein, serine palmitoyltransferase long chain base subunit 1 Protein, serine palmitoyltransferase, long chain base subunit 1 Protein, serine palmitoyltransferase, long chain base subunit 1 L homeolog Protein, SPT1 Protein, AFUA_6G00300 Protein, PAS_FragB_0040 Protein, SPTLC1 Protein, sptlc1 Protein, sptlc1.L Protein, Sptlc1 Protein

Hintergrund: Serine palmitoyltransferase (SPT). The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs (By similarity). {ECO:0000250}.

Molekulargewicht: 49.4 kDa Including tag.

UniProt: O35704